Cytoskeleton Proteins: A Purification Manual

ISBN-13: 9783642489617 / Angielski / Miękka / 2012 / 269 str.

Gerhard Isenberg

Opis

The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. Proteins are listed alphabetically and each protocol follows a common format. Thus, the manual provides a quick and easy reference to all relevant procedures for cytoskeleton protein purification.
The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No. of sequences as well as further relevant facts and practical tips are given on a separate page.

Recenzje

"In one word, a perfect book, which we recommend highly, since necessary, without any exception, to all cellular and molecular laboratories, and also to the great majority of all other laboratories." Cellular and Molecular Biology

Spis treści

A General.- I User’s Instructions.- II General Lab Equipment.- III Preparation of Buffers.- IV Ammonium Sulfate Precipitation.- V Chromatographic Fractionation.- VI Centrifugation.- VII Abbreviations.- B Purification Protocols.- I Actin and Actin-Associated Proteins.- II Tubulin and Microtubule-Associated Proteins.- III Motor Proteins.- IV Intermediate Filament Proteins.- V Plasmamembrane and Organelle-Associated Proteins.- C Suppliers List.