Culture of Hematopoietic Cells

ISBN-13: 9780471588306 / Angielski / Miękka / 1994 / 296 str.

Ian Freshney; Mary G. Freshney; Ian B. Pragnell

Opis

This guide provides a comprehensive synthesis of proven methods for the culture and experimental manipulation of haemopoietic cells. Covering a range of human and mouse cell types, and featuring step-by-step laboratory procedures, it gives detailed instructions for reagent preparation.

Spis treści

Partial table of contents:

PRIMITIVE PROGENITOR ASSAYS.

Cobblestone Area Forming Cell (CAFC) Assay (R. Ploemacher).

Assay for Murine Blast Cell Colonies (N. Katayama & M. Ogawa).

Serum–Deprived Cultures of Primary Hematopoietic Cells (G. Migliaccio & A. Migliaccio).

COLONY–FORMING ASSAYS FOR MALIGNANT CELLS.

Colony–Forming Assays for Acute Myeloid Leukemia (S. Rogers, et al.).

LONG–TERM ASSAYS AND IMMORTALIZATION.

Long–Term Culture of Human Myeloid Cells (H. Sutherland & C. Eaves).

Immortalization of Hematopoietic Cells (C. MacDonald).

PURIFICATION OF PROGENITOR CELLS.

Purification of Murine Granulocyte–Macrophage Progenitor Cells (CFU–GM) Using Counterflow Centrifugal Elutriation (S. Cooper & H. Broxmeyer).

OTHER SYSTEMS AND ASSAYS.

Culture of Human and Mouse Bone Marrow Stromal Cells (S. Perkins & R. Fleischman).

List of Suppliers.

Index.