Cryptosporidium: Methods and Protocols

ISBN-13: 9781493997503 / Angielski / Miękka / 2020 / 407 str.

Mead, Jan R.

Opis

This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.

The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

1. Cryptosporidium Diagnostic Assays: Microscopy

            Guy Robinson and Rachel M. Chalmers

 

2. Cryptosporidium Diagnostic Assays: Molecular Detection

            Guy Robinson, Kristin Elwin, and Rachel M. Chalmers

 

3. Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End Ultrafiltration

            Amy M. Kahler and Vincent R. Hill

 

4. Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation

            Michael J. Arrowood

 

5. Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay

            Jeffrey W. Priest and Delynn M. Moss

 

6. Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii

            Iwona Driskell and Roberta M. O’Connor

 

7. Cryptosporidium Genotyping for Epidemiology Tracking

            Dawn M. Roellig and Lihua Xiao

 

8. Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection

            Anson V. Koehler and Jan Šlapeta

 

9. Preparation of Cryptosporidium DNA for Whole Genome Sequencing

            Guy Robinson and Rachel M. Chalmers

 

10. Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.org

            Susanne Warrenfeltz and Jessica C. Kissinger on behalf of the EuPathDB Team

 

11. A Novel Method to Silence Genes in Cryptosporidium

            Alejandro Castellanos-Gonzalez

 

12. Use of miRNAs to Study Host Cell-Parasite Interactions

            Ji-Hao Xu, Nicholas W. Mathy, and Xian-Ming Chen

 

13. Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

            Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, and Boris Striepen

 

14. Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology

            Karine Sonzogni-Desautels, Jan R. Mead, and Momar Ndao

 

15. Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics

            Michael W. Riggs and Deborah A. Schaefer

 

16. Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water

            Jennifer L. Murphy and Michael J. Arrowood

 

17. High-Content Screening for Cryptosporidium Drug Discovery

            Melissa S. Love and Case W. McNamara

 

18. High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR

            Haili Zhang and Guan Zhu

 

19. In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds

            Nigel Yarlett, Mary Morada, Mohini Gobin, Wesley Van Voorhis, and Samuel Arnold

 

20. In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers

            Georgia Wilke, Yi Wang, Soumya Ravindran, Thaddeus Stappenbeck, William Witola, and L. David Sibley

 

21. Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium

            Daviel Cardenas, Seema Bhalchandra, Hymlaire Lamisere, Ying Chen, Xi-Lei Zeng, Sasirekha Ramani, Umesh C. Karandikar, David L. Kaplan, Mary K. Estes, and Honorine D. Ward