Cryopreservation and Freeze-Drying Protocols

ISBN-13: 9781617375286 / Angielski / Miękka / 2010 / 347 str.

John G. Day; Glyn Stacey

Opis

Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995. This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.

Recenzje

From the reviews of the second edition:

"...practical and reliable resource for the conservation and sustainable use of biological resources."-Anticancer Research

"This book is a welcome addition to the relatively sparse comprehensive reference publications on preservation methodologies which are currently available. It begins sensibly with a grounding in the principles involved in preservation, in logical and easy to follow steps. It is well laid out and covers a wide spectrum of cell/organism types in the one volume ... ." (Peter Green, Molecular Biotechnology, December, 2007)

Spis treści

Contents Preface Contributors Chapter 1 Long-term ex situ conservation of Biological Resources and the role of Biological Resource Centres Glyn N. Stacey and John G. Day Chapter 2 The Process of Freeze-drying Gerald Adams Chapter 3 Principles of Cryopreservation David E. Pegg Chapter 4 Lyophilization of Proteins Paul Matejschuk Chapter 5 Vacuum-drying and Cryopreservation of Prokaryotes Brian J. Tindall Chapter 6 Freeze-drying of Yeast Cultures Chris Bond Chapter 7 Cryopreservation of Yeast Cultures Chris Bond Chapter 8 Freeze-drying Fungi using a Shelf-freeze-drier C. Shu-hui Tan, Cor W. van Ingen and Joost A. Stalpers Chapter 9 Cryopreservation and Freeze-drying of Fungi employing Centrifugal and Shelf Freeze-drying Matthew J. Ryan and David Smith Chapter 10 Cryopreservation of Microalgae and Cyanobacteria John G. Day Chapter 11 Cryopreservation of Plant Cell Suspensions Brian W. W. Grout Chapter 12 Cryopreservation of Shoot-tips and Meristems Erica E. Benson, Keith Harding and Jason W. Johnston Chapter 13 Cryopreservation of Desiccation Tolerant Seeds Hugh W. Pritchard Chapter 14 Cryopreservation of Fish Sperm Eugeny Kopeika, Julia Kopeika and Tiantian Zhang Chapter 15 Cryopreservation of Avian Spermatozoa Graham Wishart Chapter 16 Cryopreservation of Animal and Human Cell Lines Christopher B. Morris Chapter 17 Cryopreservation of Haematopoietic Stem/Progenitor Cells for Therapeutic Use Suzanne M. Watt, Eric Austin and Sue Armitage Chapter 18 Cryopreservation of Human Embryonic Stem Cell Lines Charles J. Hunt and Paula M. Timmons Chapter 19 Cryopreservation of Primary Animal Cell Cultures Glyn N. Stacey and Stuart Dowall Chapter 20 Cryopreservation of Red Blood Cells and Platelets Andreas Sputtek Chapter 21 Cryopreservation of Mammalian Semen Mark R. Curry Chapter 22 Cryopreservation of Mammalian Oocytes Sharon J. Paynter and Barry J. Fuller Chapter 23 Cryopreservation of Mammalian Embryos Barry J. Fuller and Sharon J. Paynter Glossary