Preface Volker Gurtler and Charles Pavia 1. Sensitive methods for detection of SARS-CoV-2 RNA Xi Chen and Simin Xia 2. Treatment of COVID-19 using Chinese herbal medicine William Cho 3. Understanding how SARS-CoV-2 is evolving and its impact on COVID-19 animal models and vaccine evaluation Seshadri S. Vasan 4. Methods in machine learning to identify COVID-19 literature Anhvinh Doanvo 5. COVID-19 seasonal behavior and the mutational landscape of the SARS-CoV-2 virus Gustavo Caetano-Anolles 6. Afsar Raza Naqvi 7. CRISPR use in Diagnosis and Therapy for COVID-19 Yashpal Singh Malik, Pallavi Deol and Rahul Kaushik 8. Kuldeep Dhama 9. Recent and advanced nanotechnological strategies for COVID-19 vaccine development Chinekwu Sherridan Nwagwu 10. A review of hypersensitivity methods to measure cellular immune responses to SARS-CoV-2 Victor Matheu and Yvelise Barrios 11. Role of mRNA technology and gene silencing in manufacturing vaccines against SARS-CoV-2 Pratik Talukder 12. Hesitancy to get vaccinated against COVID-19 and how it might be overcome Charles Pavia