Correlation Force Spectroscopy for Single Molecule Measurements

ISBN-13: 9783319386409 / Angielski / Miękka / 2016 / 117 str.

Milad Radiom

Opis

This thesis addresses the development of a new force spectroscopy tool, correlation force spectroscopy (CFS) for the measurement of the properties of very small volumes of material (molecular to m³) at kHz-MHz frequency range. CFS measures the simultaneous thermal fluctuations of two closely-spaced atomic force microscopy (AFM) cantilevers. CFS then calculates the cross-correlation in the thermal fluctuations that gives the mechanical properties of the matter that spans the gap of the two cantilevers. The book also discusses development of CFS, its advantages over AFM, and its application in single molecule force spectroscopy and micro-rheology.

Spis treści

Chapter 1 - Introduction
Overview of Single Molecule Force Spectroscopy Techniques
Experiments Using Single molecule AFM
Limitations of AFM-based single molecule force spectroscopy
Summary 

Chapter 2- Correlation Force Spectroscopy 
Correlation Force Spectroscopy: Rationale 
Correlation Force Spectroscopy: Development
Laterally Offset Configuration 
Vertically Offset Configuration 
Analysis of Correlations between Two Cantilevers 
Validation of Fluctuation-Dissipation Theorem for One Cantilever 
Analysis of Thermal Fluctuations to obtain Correlations 

Chapter 3- Dynamics of Single Molecules 
Elastic Properties of Single Molecules: Worm-Like Chain and Freely-Jointed Chain Models
Hydrodynamics of Single Molecules: Dumbbell Model and Rouse Model
Internal Friction
Rouse with Internal Friction
Model of Linear Viscoelasticity of a Semiflexible Chain
Summary

Chapter 4- Microrheology with Correlation Force Spectroscopy
Existing Techniques of Rheometry
Experimental Methods
Comparison to Finite Element Analysis
Comparison to Simple Harmonic Oscillator Model
Summary

Chapter 5- Development of Colloidal Probe Correlation Force Spectroscopy: Case Study
Materials and Methods
Analysis
Results 
Discussion 
Summary 

Chapter 6- Correlation Force Spectroscopy for Single Molecule Measurements
Effect of the Distance between Cantilever Tips
Harmonic Oscillator Modeling of Vertically Offset Correlation Force Spectroscopy
Summary

Chapter 7- Single Molecule Force Spectroscopy of Dextran
Materials and Methods
Results
Stretching a Dextran Molecule
Static Force-Elongation Mode
Dynamic Correlated Fluctuations Mode
Discussion
Summary 

Chapter 8- Single Molecule Force Spectroscopy of Single-Stranded DNA
Rationale 
Introduction Materials and Methods 
Modal Analysis
Results 
Discussion
Summary

Chapter 9- Summary
An Overview of Chapters 
Future Work

Appendix I: Simple Harmonic Oscillator Model for Laterally Offset Correlation Force Spectroscopy

Appendix II: Simple Harmonic Oscillator Model for Vertically Offset Correlation Force Spectroscopy with Tethered Molecule

References

O autorze

Milad Radiom received his PhD in Chemical Engineering at Virginia Tech in 2014, after MEng and BSc in Thermal and Fluids Engineering and Mechanical Engineering respectively from Nanyang Technological University and Amirkabir University of Technology. Thereafter, he was appointed as a postdoctoral research associate in Laboratory of Colloid and Surface Chemistry, University of Geneva.  His research interests are physical chemistry of polymers, colloids and surfaces as related to single molecule force spectroscopy, micro-rheology and surface forces.

Blurb

This thesis addresses the development of a new force spectroscopy tool, correlation force spectroscopy (CFS) for the measurement of the properties of very small volumes of material (molecular to µm³) at kHz-MHz frequency range. CFS measures the simultaneous thermal fluctuations of two closely-spaced atomic force microscopy (AFM) cantilevers.  CFS then calculates the cross-correlation in the thermal fluctuations that gives the mechanical properties of the matter that spans the gap of the two cantilevers. The book also discusses development of CFS, its advantages over AFM, and its application in single molecule force spectroscopy and micro-rheology.