Coronaviruses: Methods and Protocols

ISBN-13: 9781493949212 / Angielski / Miękka / 2016 / 285 str.

Helena Jane Maier; Erica Bickerton; Paul Britton

Opis

This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field.

Spis treści

1. Coronaviruses: An Overview of Their Replication and Pathogenesis
Anthony R. Fehr and Stanley Perlman

Section I: Detection, Diagnosis and Evolution of Coronaviruses

2. Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics
Mariette F. Ducatez and Jean-Luc Guérin

3. Serological Diagnosis of Feline Coronavirus Infection by Immunochromatographic Test
Tomomi Takano and Tsutomu Hohdatsu

4. Estimation of Evolutionary Dynamics and Selection Pressure in Coronaviruses
Muhammad Munir and Martí Cortey

Section II: Propogation, Titration, and Purification of Coronaviruses

5. The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
Ruth M. Hennion

6. The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
Ruth M. Hennion and Gillian Hill

7. Isolation and Propagation of Coronaviruses in Embryonated Eggs
James S. Guy

8. Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures
Hulda R. Jonsdottir and Ronald Dijkman

9. Quantification of Infectious Bronchitis Coronaviruses by Titration in vitro and in ovo
Joeri Kint, Helena J. Maier, and Erik Jagt

10. Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis
Stuart Dent and Benjamin W. Neuman

11. Partial Purification of IBV and Subsequent Isolation of Viral RNA for Next Generation Sequencing
Sarah M. Keep, Erica Bickerton, and Paul Britton

Section III: Manipulating the Genomes of Coronaviruses

12. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses
Sarah M. Keep, Erica Bickerton, and Paul Britton

13. Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
Fernando Almazán, Silvia Márquez-Jurado, Aitor Nogales, and Luis Enjuanes

Section IV: Coronavirus Attachment and Entry

14. Protein Histochemistry Using Coronaviral Spike Proteins: Studying Binding Profiles and Sialic Acid Requirements for Attachment to Tissues
I. N. Ambepitiya Wickramasinghe, M. H. Verheije

15. Identification of Protein Receptors for Coronaviruses by Mass Spectrometry
V. Stalin Raj, Mart M. Lamers, Saskia L. Smits, Jeroen A. A. Demmers, Huihui Mou, Berend-Jan Bosch, Bart L. Haagmans

16. Single Particle Tracking Assay to Study Coronavirus Membrane Fusion
Deirdre A. Costello and Susan Daniel

Section V: Studying Virus-Host Interactions

17. Studying Coronavirus-Host Protein Interactions
Chee-Hing Yang, Hui-Chun Li, Cheng-Huei Hung, and Shih-Yen Lo

18. A Field-Proven Yeast Two-Hybrid Protocol Used to Identify Coronavirus-Host Protein-Protein Interactions
Pierre-Olivier Vidalain, Yves Jacob, Marne C. Hagemeijer, Louis M. Jones, Grégory Neveu, Jean-Pierre Roussarie, Peter J. M. Rottier, Frédéric Tangy, and Cornelis A. M. de Haan

19. Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach
Jean Kaoru Millet and Béatrice Nal

20. Transcriptome Analysis of Feline Infectious Peritonitis Virus (FIPV) Infection
Parvaneh Mehrbod, Mohammad Syamsul Reza Harun, Ahmad Naqib Shuid, Abdul Rahman Omar

21. Quantification of Interferon Signaling in Avian Cells
Joeri Kint and Maria Forlenza

22. Studying the Dynamics of Coronavirus Replicative Structures
Marne C. Hagemeijer and Cornelis A. M. de Haan

23. Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope
Philippa C. Hawes

Blurb

This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize virus replication complexes. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields.