Coronaviruses: Methods and Protocols

ISBN-13: 9781071609026 / Angielski / Miękka / 2021 / 278 str.

Helena J. Maier; Erica Bickerton

Spis treści

1. Coronaviruses: An Updated Overview of Their Replication and Pathogenesis

            Yuhang Wang, Matthew Grunewald, and Stanley Perlman

 

Part I: Coronavirus Detection, Discovery, and Evolution

 

2. Discovery of Novel Coronaviruses in Rodents

            Theocharis Tsoleridis and Jonathan K. Ball

 

3. Detection and Discovery of Coronaviruses in Wild Bird Populations

            Chantal J. Snoeck and Siamak Zohari

 

4. Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV)

            Shuetsu Fukushi

 

5. Whole Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing

            Graham L. Freimanis and Michael S. Oade

 

Part II: Propagation and Titration of Coronaviruses

 

6. Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study

            Hui Hu, Kwonil Jung, Scott P. Kenney, and Linda J. Saif

 

7. The Preparation of Chicken Kidney Cell Cultures for Virus Propagation

            Elena Lokhman, Srijana Rai, and Billy Matthews

 

8. The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation

            Giulia Dowgier and Erica Bickerton

 

9. Isolation and Propagation of Coronaviruses in Embryonated Eggs

            James S. Guy

 

10. Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures

            Mitra Gultom, Laura Laloli, and Ronald Dijkman

 

11. Quantification of Coronaviruses by Titration In Vitro and Ex Vivo

            Giulia Dowgier and Helena J. Maier

 

Part III: Manipulating the Genomes of Coronaviruses

 

12. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses

            Sarah Keep, Paul Britton, and Erica Bickerton

 

13. In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline

            Tran Thi Nhu Thao, Fabien Labroussaa, Nadine Ebert, Joerg Jores, and Volker Thiel

 

Part IV: Studying Virus-Host Interactions

 

14. Proximity Labeling for Identification of Coronavirus-Host Protein Interactions

            Philip V’kovski, Silvio Steiner, and Volker Thiel

 

15. Using Yeast to Identify Coronavirus-Host Protein Interactions

            Stuart Weston and Matthew Frieman

 

16. Determining How Coronaviruses Overcome the Interferon and Innate Immune Response

            Christopher M. Coleman

 

17. Ribopuromycylation in Coronavirus-Infected Cells

            Matthew J. Brownsword, Helena J. Maier, and Nicolas Locker

 

Part V: Imaging Coronavirus Infections

 

18. Visualizing Coronavirus Entry into Cells

            Aleksandra Milewska, Katarzyna Owczarek, Artur Szczepanski, and Krzysztof Pyrc

 

19. Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope

            Nicole Doyle and Philippa C. Hawes

Blurb

This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals.