Corneal Regeneration: Methods and Protocols

ISBN-13: 9781071606018 / Angielski / Miękka / 2021 / 250 str.

Mark Ahearne

Spis treści

1. Isolation and Culture of Corneal Stromal Stem Cells

Richard M Nagymihaly, Morten C Moe, and Goran Petrovski

2. In vitro Expansion of Corneal Endothelial Cells for Transplantation

Kim Santerre, Isabelle Xu, Mathieu Thériault, and Stéphanie Proulx

 

3. Primary Culture of Cornea-Limbal Epithelial Cells In Vitro

Finbarr O'Sullivan 

 

4. Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant

Marina López-Paniagua, Teresa Nieto-MiguelGalindo, Laura García-Posadas, Ana de la Mata, Rosa M. Corrales, Margarita Calonge, and Yolanda Diebold

 

5. A Guide to the Development of Human Cornea Organoids from Induced Pluripotent Stem Cells in Culture

James W Foster, Karl Wahlin, and Shukti Chakravarti

 

6. Gene Editing for Corneal Stromal Regeneration

Tara Moore, Connie Chao-Shern , Larry DeDionisio, Katie Christie, and Andrew M.Nesbit

 

7. Preparation and Administration of Adeno-associated Virus Vectors for Corneal Gene Delivery

Liujiang Song, Jacquelyn J. Bower, and Matthew L. Hirsch

 

8. The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea

Gaëtan Le-Bel, Pascale Desjardins, Camille Couture, Lucie Germain, and Sylvain L. Guérin

 

9. Formation of Corneal Stromal-like Assemblies using Human Corneal Fibroblasts and Macromolecular Crowding

Mehmet Gürdal, Gülinnaz Ercan, and Dimitrios I. Zeugolis

 

10. Preparation of Dried Amniotic Membrane for Corneal Repair

Andrew Hopkinson , Emily R Britchford, and Laura E Sidney

 

11. Fabrication of Corneal Extracellular Matrix Derived Hydrogels

Mark Ahearne and Julia Fernández-Pérez

 

12. Synthesis and Application of Collagens for Assembling a Corneal Implant

Elle Edin, Fiona Simpson, and May Griffith

 

13. Development and Validation of a 3D in vitro Model to Study the Chemotactic Behaviour of Corneal Stromal Fibroblasts

Evrim Ceren Kabak, Julia Fernández-Pérez, and Mark Ahearne

 

14. Femtosecond Laser Assisted Surgery for Implantation of Bioengineered Corneal Stroma to Promote Corneal Regeneration

Neil Lagali and Mehrdad Rafat

 

15. The use of Animal Models to Assess Engineered Corneal Tissue

Robert Thomas Brady and Peter W Madden

 

16. X-ray Diffraction Imaging of Corneal Ultrastructure

Keith M Meek, Andrew J. Quantock, Sally Hayes, and James Bell

Blurb

This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, how to evaluate the success of these therapies, and cell and material characterization techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Corneal Regeneration: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.