Controlled Genetic Manipulations

ISBN-13: 9781493961962 / Angielski / Miękka / 2016 / 227 str.

Alexei Morozov

Opis

This book offers basic technical information on controlled genetic manipulation, with examples of creative implementation of the methodology. Covers use of Cre-recombinase, methods for introducing genetic material into the brain, use of optogenetics and more.

Spis treści

1. Regulation of Cre Recombinase: Use of Ligand-Regulated and Dimerizable Cre for Transgenesis

            Jean-Paul Herman and Nicolas Jullien

 

2. Genetic Manipulations Using Cre and Mutant loxP Sites

            Kimi Araki and Ken-ichi Yamamura

 

3. Using Recombinant Adeno-Associated Viral Vectors for Gene Expression in the Brain

            Anke Van der Perren, Jaan Toelen, Jean-Marc Taymans, and Veerle Baekelandt

 

11. Analysis of Neuronal Circuits with Optogenetics

 

4. Using Lentiviral Vectors as Delivery Vehicles for Gene Therapy

 

11. Analysis of Neuronal Circuits with Optogenetics

            Gregory A. Dissen, Jodi McBride, Alejandro Lomniczi, Valerie Matagne, Mauricio Dorfman, Tanaya L. Neff, Francesco Galimi, and Sergio R. Ojeda

 

5. Targeting Neurological Disease with siRNA

 

11. Analysis of Neuronal Circuits with Optogenetics

            Jan Christoph Koch, Mathias Bähr, and Paul Lingor

 

6. In Utero Electroporation for Cellular Transgenesis in the Developing Mammalian Forebrain

 

11. Analysis of Neuronal Circuits with Optogenetics

            Brady J. Maher and Joseph J. LoTurco

 

7. Single-Cell Electroporation of siRNA in Primary Neuronal Cultures

 

11. Analysis of Neuronal Circuits with Optogenetics

            Masahiko Tanaka

 

8. Development and Application of Membrane-Tethered Toxins for Genetic Analyses of Neuronal Circuits

 

11. Analysis of Neuronal Circuits with Optogenetics

            Sebastian Auer and Inés Ibañez-Tallon

 

9. Genetic Methods for Anatomical Analysis of Neuronal Circuits

 

11. Analysis of Neuronal Circuits with Optogenetics

            Leah R. DeBlander, Aldis P. Weible, and Clifford G. Kentros

 

10. Functional Circuitry Analysis in Rodents Using Neurotoxins/Immunotoxins

 

11. Analysis of Neuronal Circuits with Optogenetics

            Kazuto Kobayashi, Kana Okada, and Nobuyuki Kai

            Feng Zhang, Le Cong, Garret D. Stuber, Antoine Adamantidis, and Karl Deisseroth

 

11. Analysis of Neuronal Circuits with Optogenetics

            Feng Zhang, Le Cong, Garret D. Stuber, Antoine Adamantidis, and Karl Deisseroth

Blurb

The current demand for the development of techniques for controlled genetic manipulations is driven by the anatomical and physiological complexity of the brain and by the need for experimental models that can address this complexity through selective manipulation of defined components of the system: specific neuronal populations or selected synapses.  In Controlled Genetic Manipulations, expert researchers present detailed chapters to supply basic technical information about controlled genetic manipulations and to provide examples of creative implementation of this methodology when addressing a unique biological problem. Some chapters of the book describe the most recent developments in the basic methodology, which includes use of Cre-recombinase, methods for delivery of genetic material into the brain and the use of optogenetics, whereas other chapters focus on applying these techniques to addressing particular biological questions like structural and functional mapping of neuronal circuits, analysis of specific synaptic connections, modeling or gene therapy of neurological disorders.  As part of the Neuromethods series, chapters include key implementation advice that is crucial for getting optimal results.

 

Authoritative and practical, Controlled Genetic Manipulations serves as a valuable guide to a variety of techniques, provided by many of the pioneers of the approaches.