Contemporary Research Methods in Neuroanatomy: Proceedings of an International Conference Held at the Laboratory of Perinatal Physiology, San Juan, Pu

ISBN-13: 9783642859885 / Angielski / Miękka / 2012 / 388 str.

Walle J. H. Nauta; Sven O. E. Ebbesson

Opis

As a source of information on neuroanatomical research methods this Volume is not without precedent. In 1957, at the initiative of Dr. W. F. Windle, a conference was held at the National Institutes of Health, the proceedings of which, edited by Dr. Windle and published by C. C. Thomas under the title "New Research Tech niques of Neuroanatomy," rapidly became something like a standard reference in the field of Neuromorphology. The present editors were emboldened to seek support for a second expose of contemporary research methods in Neuroanatomy by the success of this earlier publication, as well as by the consideration that the years elapsed since its appearance have been, perhaps, more productive of new research methods and strategies in Neuroanatomy than were any dozen consecutive years since the golden decades of the 1870's and 1880's. The decision, which methods to include in this conference, has been a difficult one. For reasons of space alone it would have been impossible to do equal justice to techniques approaching the brain as a neuronal system, the brain as a tissue, or the neuron as a cell. As a brief inspection of the contents of this volume will show, the weight of choice fell upon the first of these alternatives. The reader will find, further more, that not all of the book is devoted to new methods in the strict sense."

Spis treści

The Rapid Golgi Method. Indian Summer or Renaissance?.- The Golgi Method. A Tool for Comparative Structural Analyses.- The Golgi-Cox Technique.- The Fixation of Central Nervous Tissue and the Analysis of Electron Micrographs of the Neuropil, with Special Reference to the Cerebral Cortex.- Light-and Electron-Microscopical Studies of Normal and Degenerating Axons.- Selective Silver-Impregnation of Degenerating Axoplasm.- The Selective Silver-Impregnation of Degenerating Axons and their Synaptic Endings in Nonmammalian Species.- Bridging the Gap between Light and Electron Microscopy in the Experimental Tracing of Fiber Connections.- Neuronal Changes Central to the Site of Axon Transsection. A Method for the Identification of Retrograde Changes in Perikarya, Dendrites and Axons by Silver-Impregnation.- Electron Microscopy of Golgi Preparations for the Study of Neuronal Relations.- Anterograde and Retrograde Transneuronal Degeneration in the Central and Peripheral Nervous System.- Autoradiographic Methods and Principles for Study of the Nervous System with Thymidine-H3.- Fluorescence Microscopy in Neuroanatomy.- Methods for the Counting of Neurons.- Subject-Index.