Confocal Raman Microscopy

ISBN-13: 9783642266829 / Angielski / Miękka / 2013 / 292 str.

Thomas Dieing; Olaf Hollricher; Jan Toporski

Opis

Confocal Raman Microscopy is a relatively new technique that allows chemical imaging without specific sample preparation. By integrating a sensitive Raman spectrometer within a state-of-the-art microscope, Raman microscopy with a spatial resolution down to 200nm laterally and 500nm vertically can be achieved using visible light excitation. Recent developments in detector and computer technology as well as optimized instrument design have reduced integration times of Raman spectra by orders of magnitude, so that complete images consisting of tens of thousands of Raman spectra can be acquired in seconds or minutes rather than hours, which used to be standard just one decade ago. The purpose of this book is to provide the reader a comprehensive overview of the rapidly developing field of Confocal Raman Microscopy and its applications.

Spis treści

Part I: Theory.- Confocal Microscopy.- Introduction into the Fundamentals of Raman Spectroscopy.- Raman Equipment.- Software Requirements and Data Analysis in Confocal Raman Microscopy.- Part II: Applications.- Identification of Pathogens.- Imaging of Individual Carbon Nanotubes and Isolated Graphene Sheets.- Raman Microscopy in Geosciences.- Raman Microscopic Imaging of Cells and Applications Monitoring the Uptake of Drug Delivery Systems.- Confocal Micro-Raman Spectroscopy: A Tool for the Allocation of Organic and Inorganic Components in Calcified Biominerals.- Pharmaceutical Applications of Confocal Raman Microscopy.- Applications of Confocal Raman Microscopy in Pharmaceutical Development.- Characterization of Therapeutic Coatings on Medical Devices.- Raman Imaging of Plant Cell Walls.- Confocal Raman Imaging of Polymeric Materials.- Stress Measurement by Means of Raman Microscopy