Confocal Microscopy: Methods and Protocols

ISBN-13: 9781617370625 / Angielski / Miękka / 2010 / 446 str.

Stephen W. Paddock

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Recenzje

"This book is an essential reference for anyone who is involved in the production of laser scanning confocal (LSCM) images. . . Exceptionally useful information on a great variety of methodologies that can be employed with LSCM is provided. . . In consideration of the vast quantity of useful information on all aspects of LSCM that is presented in this volume, and the fact that it is the first comprehensive book on this topic, it is highly recommended."-Quarterly Review of Biology

"...almost every research institute has a confocal microscope, and many are developing much larger multi-user biomedical imaging facilities. The publication of this collection of methods and protocols for confocal microscopy is therefore very timely...The tips and tricks used on the various systems should prove useful to a diverse range of confocal microscope users and would be a beneficial reference volume for any imaging unit and reasonable value for money."-Cell Biology International

" This is an excellent source for anyone who wants to explore techniques in confocal microscopy. It is a practical manual with many gems that will help both new and experienced users. The principles are efficiently and accessibly explained and there is a useful chapter on fluorescent probes...there is a wealth of ideas here waiting to be applied to prokaryotic biology and many would be equally useful for simple fluorescence rather than confocal studies. I would certainly recommend it to any lab that uses fluorescence microscopy extensively."-Microbiology Today

"...The texts are aimed at the biological user...for the user for whom they are intended, these essays contain a wealth of down-to-earth practical information. The book is well-produced and contains colour illustrations..."-Ultramicroscopy

"...excellent information as an introductory basis for confocal microscopy."-Cellular and Molecular Biology

Spis treści

An Introduction to Confocal Imaging, Stephen W. Paddock. Practical Considerations for Collecting Confocal Images, David Carter. Fluorescent Probes for Confocal Microscopy, Christopher Cullander. Imaging Gene Expression Using Antibody Probes, Nadean L. Brown. Single and Double FISH Protocols for Drosophila, Sarah C. Hughes and Henry M. Krause. Confocal Microscopy of Plant Cells, Carol L. Wymer, Alison F. Bevan, Kurt Boudonck, and Clive W. Lloyd. Preparation of Yeast Cells for Confocal Microscopy, Audrey Atkin. Confocal Methods for C. elegans, Sarah L. Crittenden and Judith Kimble. Imaging Sea Urchin Fertilization, Jon M. Holy. Imaging Immunolabelled Drosophila Embryos by Confocal Microscopy, James Langeland. Confocal Microscopy on Xenopus laevis Oocytes and Embryos, Denise L. Robb and Chris Wylie. Analyzing Morphogenetic Cell Behaviors in Vitally-Stained, Zebrafish Embryos, Mark S. Cooper, Leonard A. D'Amico, and Clarissa A. Henry. Confocal Imaging of Living Cells in Intact Embryos, Paul M. Kulesa and Scott E. Fraser. Live Confocal Analysis with Fluorescently Labeled Proteins, Helen Francis-Lang, Jonathan Minden, William Sullivan and Karen Oegema. Live Imaging with Green Fluorescent Protein, Jim Haseloff, Emma-Louise Dormand, and Andrea H. Brand. Fluorescent Calcium Indicators: Subcellular Behavior and Use in Confocal Imaging, Donald M. O'Malley, Barry J. Burbach, and Paul R. Adams. Intracellular pH and pCa Measurement, Michal Opas and Ewa Dziak. Measuring Dynamic Cell Volume In Situ by Confocal Microscopy, Rachel J. Errington and Nick S. White. Imaging Thick Tissues with Confocal Microscopy, Delphine Imbert, Janet Hoogstraate, Emmaline Marttin, and Chris Cullander. Measurement in the Confocal Microscope, Guy Cox. Presentation of Confocal Images, Georg Halder and Stephen Paddock. The Preparation of Stereoscopic 3D Illustrations of Confocal Data Sets for Publications and Slides, Gabriel G. Martins, Alan T. Stonebraker, and Robert G. Summers. Traditional Text-Based Databases and Image Gallery Databases, Harvey Karten. Morphing Confocal Images and Producing Digital Movies, Eric Hazen.Index.

Blurb

In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish.

Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.