Confocal Microscopy: Methods and Protocols

ISBN-13: 9781071614013 / Angielski / Twarda / 2021 / 363 str.

Joseph Brzostowski; Haewon Sohn

Spis treści

1. Advances in Confocal Microscopy and Selected Applications

W. Matt Reilly and Christopher J. Obara

 

2. Choosing Fluorescent Probes and Labeling Systems

Jacoby Morris and George H. Patterson

 

3. General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy

Joseph A. Brzostowski

 

4. Microfabricated devices for confocal microscopy on biological samples

Nicole Y. Morgan

          

5. Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging

Xufeng Wu and John A. Hammer

 

6. High-resolution multicolor imaging of mitochondria in lymphocytes

Munir Akkaya, Pietro Miozzo, and Margery G. Smelkinson

 

7. Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution    Microscopy

Paul Tillberg

 

8. Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy

Abhijit Ashok Ambegaonkar and Haewon Sohn

 

9. Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight 

Sara Hernández-Pérez, Marika Vainio, Vid Šuštar, and Pieta K. Mattila

                                                                                                                                    

10. Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopy in the Model Organism Dictyostelium discoideum

Xuehua Xu, Joseph Brzostowski, Sharmila Ramachandra, Smit Bhimani, Yan You, and Tian Jin          

 

11. Imaging GPCR-mediated Signal Events Leading to Chemotaxis and Phagocytosis

Miao Pan and Tian Jin

 

12. High-throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains

Mojca Mattiazzi Usaj, Dara S Lo, Ben T Grys, and Brenda J Andrews

 

13. Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy

Billur Akkaya, Olena Kamenyeva, Juraj Kabat, and Ryan Kissinger

 

14. Studying Neuronal Biology Using Spinning Disc Confocal Microscopy

Javier Manzella-Lapeira, Joseph Brzostowski, and Jenny Serra-Vinardell

     

15. Method for Acute Intravital Imaging of the Large Intestine in Live Mice

Erreni Marco, Doni Andrea and Roberto Weigert

 

16. Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism

Javier Manzella-Lapeira and Joseph Brzostowski

 

17. Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry

Greg Alspaugh, Branden Roarke, Alexandra Chand, Rozhin Penjweini, Alessio Andreoni, and Jay R. Knutson

 

18. FLIM Imaging for Metabolic Studies in Live Cells

Heejun Choi

 

19. A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM) 

Alexander Zhovmer and Christian A. Combs

Blurb

This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types.  Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Confocal Microscopy: Methods and Protocols aims to ensure successful results in the further study of this vital field.