1. The viral replication organelles within cells studied by electron microscopy Martin Sachse, Isabel Fernández de Castro, Raquel Tenorio and Cristina Risco 2. Structures of enveloped virions determined by cryogenic electron microscopy and tomography Robert Stass, Weng M. Ng, Young Chan Kim and Juha T. Huiskonen 3. CryoEM reconstruction approaches to resolve asymmetric features Daniel J. Goetschius, Hyunwook Lee and Susan Hafenstein 4. Structural insights into coronavirus entry Maria Alejandra Tortorici and David Veesler 5. Structural analysis of pleomorphic and asymmetric viruses using cryo-electron tomography and subtomogram averaging Martin Obr and Florian KM Schur 6. The application of atomic force microscopy for viruses and protein shells: Imaging and spectroscopy Pedroa J. de Pablo 7. Structural mass spectrometry goes viral Jasmin Dülfer, Alan Kadek, Janine-Denise Kopicki, Boris Krichel and Charlotte Uetrecht 8. Illuminating the virus life cycle with single-molecule FRET imaging Maolin Lu, Xiaochu Ma and Walther Mothes 9. Polyhedra, spindles, phage nucleus and pyramids: Structural biology of viral superstructures Fasséli Coulibaly