Comparative Genomics of Salmonella spp using PFGE : Pulsed Field Gel Electrophoresis

ISBN-13: 9786202029070 / Angielski / Miękka / 2017 / 104 str.

Laxmikant Kamble; Irshad Ahmed; Ramees Muhammad

Opis

Salmonella spp. reported to be one of the most important food pathogen studied worldwide. Salmonella causes typhoidal and non-typhoidal diseases in humans and animals. The genetic relatedness of the isolates was correlated using bioinformatics software, Applied Math. A standard salmonella isolation method was used in the bacterial isolation. Colonies from microbial plates were confirmed using MicroSEQ Salmonella spp. Detection Kits, and Step One Plus RT PCR Machine from Applied Biosystems. A genetic dendrogram was generated using Applied Math software from the PFGE gels generated by Xbal digestion of the strains and using Salmonella braenderup (H9812) as reference strain. The technique was helpful in correlating the bacterial strains from poultry and environmental samples.

O autorze

Kamble, LaxmikantDr. Laxmikant H. Kamble is Associate Professor (Biochemistry) in DST-FIST Sponsored School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded MS India. Dr. Ramees KM is Ph. D. in Biotechnology and Dr. Irshad Ahmed is group leader in Central Food Laboratories,Ministry of Public Health, Qatar.