Combinatorial Library: Methods and Protocols

ISBN-13: 9781489938992 / Angielski / Miękka / 2013 / 383 str.

Lisa B. English

Opis

The continued successes of large- and small-scale genome sequencing projects are increasing the number of genomic targets available for drug d- covery at an exponential rate. In addition, a better understanding of molecular mechanisms-such as apoptosis, signal transduction, telomere control of ch- mosomes, cytoskeletal development, modulation of stress-related proteins, and cell surface display of antigens by the major histocompatibility complex m- ecules-has improved the probability of identifying the most promising genomic targets to counteract disease. As a result, developing and optimizing lead candidates for these targets and rapidly moving them into clinical trials is now a critical juncture in pharmaceutical research. Recent advances in com- natorial library synthesis, purification, and analysis techniques are not only increasing the numbers of compounds that can be tested against each specific genomic target, but are also speeding and improving the overall processes of lead discovery and optimization. There are two main approaches to combinatorial library production: p- allel chemical synthesis and split-and-mix chemical synthesis. These approaches can utilize solid- or solution-based synthetic methods, alone or in combination, although the majority of combinatorial library synthesis is still done on solid support. In a parallel synthesis, all the products are assembled separately in their own reaction vessels or microtiter plates. The array of rows and columns enables researchers to organize the building blocks to be c- bined, and provides an easy way to identify compounds in a particular well.

Recenzje

"...an excellent work that covers many important aspects, gives easy access to literature for further study, and contains much practical advice. As it describes many different tools and techniques it is especially suitable for beginners in combinatorial chemistry, and deserves to be added to the list of well presented works in this field." - Angewandte Chemie

"The book provides a wealth of excellent reference material and it is a worthwhile addition to the library of most medicinal chemists." -Journal of Medicinal Chemistry

Spis treści

Part I. Library Synthesis and Quality Control Using a Noncovalent Protection Strategy to Enhance Solid-Phase Synthesis Fahad Al-Obeidi, John F. Okonya, Richard E. Austin, and Dan R. S. Bond Quality Control of Solid-Phase Synthesis by Mass Spectrometry Jean-Louis Aubagnac, Robert Combarieu, Christine Enjalbal, and Jean Martinez Preparation of Encoded Combinatorial Libraries for Drug Discovery Tao Guo and Doug W. Hobbs Simple Tools for Manual Parallel Solid Phase Synthesis Viktor Krchnák and Andrew Burritt Cleavage of Compounds from Solid Phase by Gaseous Reagents Viktor Krchnák Synthesis of DNA-Binding Polyamides: Robust Solid-Phase Methods for Coupling Heterocyclic Aromatic Amino Acids Peter O. Krutzik and A. Richard Chamberlin The Preparation of Phenyl-stilbene Derivatives Using the Safety Catch Linker Amy Lew and A. Richard Chamberlin Host-Guest Chemistry: Combinatorial Receptors Brian R. Linton Automated Structure Verification of Small Molecules Libraries Using 1D and 2D NMR Techniques Gérard Rossé, Peter Neidig, and Harald Schröder Rapid Liquid-Phase Combinatorial Synthesis of Heterocyclic Libraries Chung-Ming Sun Soluble Polymer-Supported Methods for Combinatorial and Organic Synthesis Paul Wentworth, Jr. and Carsten Spanka Analytical Methods for Optimization and Quality Control of Combinatorial Synthesis Bing Yan, Liling Fang, Mark M. Irving, Jiang Zhao, Diana Liu, Gianine M. Figliozzi, Frank Woolard, and Clinton A. Krueger Part II. Library Purification and Screening Resolving Racemic Mixtures Using Parallel Combinatorial Libraries Tingyu Li, Yan Wang, and Louis H. Bluhm Ligand Libraries for the Extraction of Metal Ions: Dynamic Combinatorial and High-Throughput Screening Methods Seema Choudhary and Janet R.Morrow Automated Liquid-Liquid Extraction and Ion-Exchange Solid-Phase Extraction for Initial Purification Sean X. Peng and Charles Henson On-Bead and Solution Screening Approaches for Genomically Derived Targets: Discovery of Surrogate Ligands and Substrates Using Combinatorial Chemistry Libraries David S. Thorpe, Gérard Rossé, Helen Yeoman, Sydney Wilson, Patti Willson, Greg Harlow, Anna Robinson, and Kenneth F. Wertman Part III. Computational Library Design Design of Virtual Combinatorial Libraries Alex M. Aronov Approaches to Library Design for Combinatorial Chemistry Stefan Güssregen, Bernd Wendt, and Mark Warne Reagent-Based and Product-Based Computational Strategies in Library Design Eric A. Jamois Designing Combinatorial Libraries for Efficient Screening John I. Manchester and David S. Hartsough Application of Neural Networks to Large Dataset QSAR, Virtual Screening, and Library Design David A. Winkler and Frank R. Burden Index

Blurb

By significantly increasing the number of targets available for drug discovery, the Human Genome and Proteome projects have made the use of combinatorial libraries essential to developing and optimizing drug candidate molecules more rapidly. Lisa English and a panel of expert researchers have collected in Combinatorial Library Methods and Protocols a novel series of computational and laboratory methods for the design, synthesis, quality control, screening, and purification of combinatorial libraries. Here the reader will find cutting-edge techniques for the preparation of encoded combinatorial libraries, for the synthesis of DNA-binding polyamides, and for combinatorial receptors. There are also state-of-the-art methods for computational library design, quality control by mass spectrometry, and structure verification using 1D and 2D NMR. A variety of well-known computational approaches are provided to meet the information management challenge of multiple biological assays. Each readily reproducible technique includes detailed step-by-step instructions and helpful notes on troubleshooting and avoiding pitfalls.
Timely and highly practical, Combinatorial Library Methods and Protocols makes available for all drug discovery researchers all the powerful combinatorial chemistry tools that are increasing the number of candidate compounds and speeding the process of drug discovery and development today.