Cloning and expression of human insulin gene in baking yeast

ISBN-13: 9783659418341 / Angielski / Miękka / 2015 / 220 str.

Mossawi Rafid

Opis

World free of the devastation of diabetes is not only an admirable vision, but is a vital necessity. The discovery and isolation of insulin in 1921, by Banting and Best, paved the way for the development of a therapy which has saved the life of many diabetic patients. Insulin was the first protein to be sequenced in 1951, marking an important milestone in molecular biology. In 1976, insulin became the first recombinant drug - obtained using newly developed genetic engineering techniques - to be used for therapy in humans . Many proteins such as human insulin cannot be obtained from their natural sources in suitable quantities because of their low abundance or difficulty of purification by conventional methods. One solution is the production of recombinant human proteins in hetero-logous prokaryotic cell systems such as yeast mediated by suitable and powerful expression vectors. Identification of a simple eukaryotic system depend on the expression level, quality of biomolecules, safety, manipulation of genetic system and finally the cost of production

O autorze

Dr. Rafid abdulwahid Al-Mossawi obtained his PhD and Msc degree in genetic engineeringfrom college of science, department of biotechnology-Baghdad University - in 2014. He worked on Pichia pastoris genetic egineering, DNA,RNA isolation from tissues, protein purification and recombinant DNA technology. He joined as Lecturers in Biochemical engineering department, Al-khwarizimi college of engineering, Baghdad University since 2002 till now.