Clinical Applications of PCR

ISBN-13: 9781493933587 / Angielski / Twarda / 2016 / 191 str.

Rajyalakshmi Luthra; Rajesh R. Singh; Keyur P. Patel

Opis

This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routine clinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Clinical Applications of PCR, Third Edition serves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.

Recenzje

"This multi-author treatise reviews the clinical applications of PCR in various fields of medicine stressing its importance for genetic analysis. It has 16 chapters, each with color illustrations and references. Recommended for researchers in biology and hospital laboratories." (Pediatric Endocrinology Reviews (PER), Vol. 14 (3), March, 2017)

Spis treści

1. A Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care

            Alain R. Thierry

 

2. COLD-PCR: Applications and Advantages

            Zhuang Zuo and Kausar J. Jabbar

 

3. PCR-Based Detection of DNA Copy Number Variation

            Meenakshi Mehrotra

 

4. Emulsion PCR: Techniques and Applications

            Rashmi Kanagal-Shamanna

 

5. Digital PCR: Principles and Applications

            Rashmi Kanagal-Shamanna

 

6. Quantitative PCR for Plasma Epstein-Barr Virus Loads in Cancer Diagnostics

      

      Sanam Loghavi

 

7. High Resolution Melt Curve Analysis in Cancer Mutation Screen

            Meenakshi Mehrotra and Keyur P. Patel

 

8. Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutations

            Khedoudja Nafa, Meera Hameed, and Marie E. Arcila

 

9. Genotyping of Frequent Mutations in Solid Tumors by PCR-Based Single-Base Extension and MassARRAY Analysis

            Alberto Paniz Mondolfi and Rajesh R. Singh

 

10. Microfluidics-Based PCR for Fusion Transcript Detection

            Hui Chen

 

11. Polymerase Chain Reaction Diagno

sis of Leishmaniasis: A Species-Specific Approach

  &n

bsp;         Eglys González-Marcano, Hirotomo Kato, Juan Luis Concepción, María Elizabeth Márquez, and Alberto Paniz-Mondolfi

 

12. Detection of Trypanosoma cruzi by Polymerase Chain Reaction

            María Elizabeth Márquez, Juan Luis Concepción, Eglys González-Marcano, and Alberto Paniz-Mondolfi

 

13. PCR Techniques in Next-Generation Sequencing

            Rashmi S. Goswami

 

14. Single Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics

            Chi Young Ok, Rajesh R. Singh, and Alaa A. Salim

 

15. Quantitative Real Time PCR: Recent Advances

            Charanjeet Singh and Sinchita Roy-Chowdhuri

 

16. PCR Techniques in Char

acterizing DNA Methylation

            Khalida Wani and Kenneth D. Aldape