Circulating Tumor Cells: Methods and Protocols

ISBN-13: 9781493971435 / Angielski / Twarda / 2017 / 306 str.

Mark Jesus Magbanua; John Park

Opis

This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Circulating Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.

Spis treści

Dedication…
Acknowledgement…
Foreword…
Preface…
Table of Contents…
Contributing Authors…

 

1.       Microfluidic Capture and Multiplex Immunofluorescence of Circulating Tumor Cells to Identify Cancer Origin
Chian-Hui Lai and Ying-Chih Chang

2.       Microfluidic Separation of Circulating Tumor Cells Based on Size and Deformability
Emily S. Park, Simon P. Duffy, and Hongshen Ma

3.       A Novel Microfluidic Device for Isolation of Circulating Tumor Cells from Pancreatic Cancer Blood Samples
Jose I. Varillas, Kangfu Chen, Jinling Zhang, Thomas J. George, Jr., and Z. Hugh Fan

4.       Microfluidic-Based Enrichment and Retrieval of Circul

ating Tumor Cells for RT-PCR Analysis
Priya Gogoi, Saedeh Sepehri, Will Chow, Kalyan Handique, and Yixin Wang

5.       Micro-Scale Laminar Vortices for High-Purity Extraction and Release of Circulating Tumor Cells
Soojung Claire Hur, James Che, and Dino Di Carlo

6.       Separable Bilayer Microfiltration Device for Viable Label-free Enrichment of Circulating Tumor Cells
Sijie Hao, Merisa Nisic, Hongzhang He, Yu-Chong Tai, and Si-Yang Zheng

7.       Microfilter-Based Capture and Release of Viable Circulating Tumor Cells
Siddarth Rawal, Zheng Ao, Ram H. Datar, and Ashutosh Agarwal

8.       Fourier Ptychographic Microscopy for Rapid, High Resolution Imaging of Circul

ating Tumor Cells Enriched by Microfiltration
Anthony Williams, Jaebum Chung, Changhuei Yang, and Richard J. Cote

9.       Automated Microfluidic Filtration and Immunocytochemistry Detection System for Capture and Enumeration of Circulating Tumor Cells and Other Rare Cell Populations in Blood
Michael Pugia, Mark Jesus M. Magbanua, and John W. Park

10.   Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells
Marianne Oulhen, Emma Pailler, Vincent Faugeroux, and Françoise Farace

11.   Negative Enrichment and Isolation of Circulating Tumor Cells for Whole Genome Amplification
Nisha Kanwar and Susan J. Done

12.   Capture and Genetic Analysis of Circulating Tumor

Cells Using a Magnetic Separation Device (Magnetic Sifter)
Chin Chun Ooi, Seung-min Park, Dawson J. Wong, Sanjiv S. Gambhir, and Shan X. Wang

13.   RareCyte® CTC Analysis Step 1:  AccuCyte® Sample Preparation for the Comprehensive Recovery of Nucleated Cells from Whole Blood
Arturo B. Ramirez, Lance U’Ren, Danny Campton, David Stewart, Joshua J. Nordberg, Jackie L. Stilwell, and Eric P. Kaldjian

14.   RareCyte ® CTC Analysis Step 2:  Detection of Circulating Tumor Cells by CyteFinder® Automated Scanning and Semi-Automated Image Analysis 
Jeffrey L. Werbin, Joshua J. Nordberg, Jay Tzucker, Paulina Varshavskaya, Jackie L. Stilwell, and Eric P. Kaldjian

15.   RareCyte ® CTC Analysis Step 3:  Using the CytePicker® Module for Individual Cell Retrieval and Subsequent Whole

Genome Amplification of Circulating Tumor Cells for Genomic Analysis
Jackie L. Stilwell, Paulina Varshavskaya, Jeffrey L. Werbin, Joshua J. Nordberg, Arturo B. Ramirez, Steve Quarre, Jay Tzucker, Jennifer Chow, Brennan Enright, and Eric P. Kaldjian

16.   Enumeration, Dielectrophoretic Capture, and Molecular Analysis of Circulating Tumor Cells
Stephanie S. Yee and Erica L. Carpenter

17.  

18.   Flow Cytometric Detection of Circulating Tumor Cells Using a Candidate Stem Cell Marker, p75 Neurotrophin

Receptor (p75NTR)<

19.   Multispectral Imaging Analysis of Circulating Tumor Cells in Negatively-Enriched Peripheral Blood Samples
Brandon Miller, Maryam Lustberg, Thomas A. Summers, and Jeffrey J Chalmers

20.   Fiber-Optic Array Scanning Technology (FAST) for Detection and Molecular Characterization of Circulating Tumor Cells
Zheng Ao and Xiaohe Liu

21.   A Non-Invasive and Real-Time Method for Circulating Tumor Cell Detection: In Vivo Flow Cytometry
Xunbin Wei, Jian Zhou, Xi Zhu, Xinrong Yang, Ping Yang, and Qiyan Wang

22.   EpCAM-Independent Enrichment and Detection of Viable CTCs Using the EPISPOT As

say
Alexandra Soler, Laure Cayrefourcq, Martine Mazel, and Catherine Alix-Panabières

23.   Utilizing Matrigel Transwell Invasion Assay to Detect and Enumerate Circulating Tumor Cells
Xingtong Liu and Xiangwei Wu

24.   Circulating Tumor Cells: Markers and Methodologies for Enrichment and Detection
M.J. Alvarez Cubero, J.A. Lorente, I. Robles-Fernandez, A. Rodriguez-Martinez, J.L. Puche, and M.J. Serrano