Chromosome Architecture: Methods and Protocols

ISBN-13: 9781493936298 / Angielski / Twarda / 2016 / 290 str.

Mark C. Leake

Opis

This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the understanding of chromosome architecture. Chromosome Architecture: Methods and Protocols guides readers through cutting-edge interdisciplinary methods which allow for an understanding of architecture of chromosomes with exceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromosome Architecture: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Spis treści

New Advances in Chromosome Architecture.- Single Molecule Narrowfield Microscopy Of Protein-DNA Binding Dynamics In Glucose Signal Transduction Of Live Yeast Cells.- Single-Molecule Imaging to Characterise the Transport Mechanism of the Nuclear Pore Complex.- Using Fluorescence Recovery after Photobleaching (FRAP) To Study Dynamics Of The Structural Maintenance Of Chromosome (SMC) Complex In Vivo.- Imaging DNA Structure by Atomic Force Microscopy.- Investigating Bacterial Chromosome Architecture Using Epifluorescence Microscopy.- Transverse Magnetic Tweezers Allowing Coincident Epi-Fluorescence Microscopy On Horizontally Extended DNA.- Probing Chromosome Dynamics in Bacillus Subtilis.- Systems Biology Approaches for Understanding Genome Architecture.- In Vivo And In Situ Replication Labeling Methods For Super-Resolution Structured Illumination Microscopy Of Chromosome Territories And Chromatin Domains.- DNA-Protein Interactions Studied Directly Using Single Molecule Fluorescence Imaging Of Quantum Dot Tagged Proteins Moving On DNA Tightropes.- Escherichia Coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis.- Bacterial Chromosome Dynamics by Locus Tracking In Fluorescence Microscopy.- Biophysical Characterization of Chromatin Remodelling Protein CHD4.- Atomistic Molecular Dynamics Simulations of DNA Minicircle Topoisomers: A Practical Guide to Setup, Performance and Analysis.- Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells.- Studying the Dynamics of Chromatin Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy.- Intra Nuclear Single Particle Tracking (I-SPT) To Reveal the Functional Architecture Of Chromosomes.- Visualizing Bacillus Subtilis during Vegetative Growth and Spore Formation.