Chromosomal Mutagenesis

ISBN-13: 9781617378331 / Angielski / Miękka / 2010 / 236 str.

Greg Davis; Kevin J. Kayser

Opis

Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Chromosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types."

Recenzje

From the reviews:

"I looked at Chromosomal Mutagenesis from the perspective of a geneticist ... . it serves as a potentially useful text. ... well written and protocols appear very detailed with good explanation that goes well beyond what one can glean from most published papers. ... On the whole, for anyone either thinking of moving into a field where chromosomal mutagenesis is not so straightforward, or looking for ideas by learning how others have solved related problems, this book could serve as a very useful resource." (Alistair Goldman, Microbiology Today, May, 2008)

Spis treści

Contents Preface Contributors Biallelic Gene Knockouts in Chinese Hamster Ovary Cells Naoko Yamane-Ohnuki, Kazuya Yamano, and Mitsuo Satoh Highly Proficient Gene Targeting by Homologous Recombination in the Human pre-B Cell Line Nalm-6 Noritaka Adachi, Aya Kurosawa, and Hideki Koyama Robust Cell Line Development Using Meganucleases Jean-Pierre Cabaniols and Frédéric Pâques Design and Testing of Zinc Finger Nucleases for Use in Mammalian Cells Matthew Porteus Gene Targeting in Drosophila and C. elegans with Zinc-finger Nucleases Dana Carroll, Kelly J. Beumer, J. Jason Morton, Ana Bozas and Jonathan K. Trautman Orpheus Recombination: A Comprehensive Bacteriophage System for Murine Targeting Vector Construction by Transplacement Knut Woltjen, Kenichi Ito, Teruhisa Tsuzuki, and Derrick E. Rancourt Transposon Mediated Mutagenesis in Somatic Cells: Identification of transposon-genomic DNA junctions David A. Largaespada and Lara S. Collier Insertional Mutagenesis of the Mouse Germline with Sleeping Beauty Transposition Junji Takeda, Zsuzsanna Izsvák, and Zoltán Ivics Conditional Gene Trapping Using the FLEx System Thomas Floss and Frank Schnütgen Steps Towards Targeted Insertional Mutagenesis with Class II Transposable Elements Sareina Chiung-Yuan Wu, Kommineni J. Maragathavally, Craig J. Coates and Joseph M. Kaminski Targeting integration of the Saccharomyces Ty5 retrotransposon Troy L. Brady, Clarice L. Schmidt and Daniel F. Voytas Site-Specific Chromosomal Integration Mediated by f C31 Integrase Annahita Keravala and Michele P. Calos Triplex-Mediated Gene Modification Erica B. Schleifman, Joanna Y. Chin, and Peter M.Glazer Allelic exchange of unmarked mutations in Mycobacterium tuberculosis. Martin S. Pavelka, Jr. Mycobacterial Recombineering Julia C. van Kessel and Graham F. Hatfull Chromosomal Engineering of Clostridium perfringens Using Group II Introns Phalguni Gupta and Yue Chen

Blurb

Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation.  In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms.  Following the format of the highly successful Methods in Molecular Biology™ format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Chomosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types.