Chromosomal Mutagenesis

ISBN-13: 9781493955411 / Angielski / Miękka / 2016 / 333 str.

Shondra M. Pruett-Miller

Opis

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols.

Spis treści

1. Genome Editing by Targeted Chromosomal Mutagenesis

            Dana Carroll

 

2. ­piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells

            Stephen J. Pettitt, E-Pien Tan, and Kosuke Yusa

 

3. Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy

            Jonathan M. Geisinger and Michele P. Calos

 

4. Genome Mutagenesis Using Synthetic Triplex-Forming Molecules and Recombinagenic DNA

            Faisal Reza and Peter M. Glazer

 

5. Genome Engineering Using Adeno-Associated Virus (AAV)

            Rob Howes and Christine Schofield

 

6. Engineering of Customized Meganucleases Via In Vitro Compartmentalization and In Cellulo Optimization

            Ryo Takeuchi, Michael Choi, and Barry L. Stoddard

 

7. Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform

            Tomas Cermak, Colby G. Starker, and Daniel F. Voytas

 

8. Ligation-Independent Cloning (LIC) Assembly of TALENs

            Jonathan L. Schmid-Burgk, Tobias Schmidt, and Veit Hornung

 

9. Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications

            Sandrine Boissel and Andrew M. Scharenberg

 

10. Genome Engineering Using CRISPR-Cas9 System

            Le Cong and Feng Zhang

 

11. Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases

            Shondra M. Pruett-Miller and Gregory D. Davis

 

12. Endogenous Gene Tagging with Fluorescent Proteins

            John Fetter, Andrey Samsonov, Nathan Zenser, Fan Zhang, Hongyi Zhang, and Dmitry Malkov

 

13. Silencing Long Non-Coding RNAs with Genome-Editing Tools

            Tony Gutschner

 

14. Gene Editing Using ssODNs with Engineered Endonucleases

            Fuqiang Chen, Shondra M. Pruett-Miller, and Gregory D. Davis

 

15. Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases

            Kunitoshi Chiba and Dirk Hockemeyer

 

16. Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells

            Matthew Porteus

 

17. Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models

            Victoria M. Bedell and Stephen C. Ekker

 

18. Creating Knockout and Knockin Rodents Using Engineered Endonucleases Via Direct Embryo Injection

            Takehito Kaneko and Tomoji Mashimo

 

19. Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains

            Takehito Kaneko

Blurb

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and fully updated, Chromosomal Mutagenesis, Second Edition aims to help speed scientific discovery and aid in the next advances in the field.