Chromatographic Methods

ISBN-13: 9789401083164 / Angielski / Miękka / 2011 / 414 str.

A. Braithwaite

Spis treści

1 Introduction.- 1.1 Introduction to chromatography.- 1.2 Historical aspects of chromatography.- 1.3 Classification of chromatographic methods.- References.- 2 Theoretical considerations.- 2.1 Factors influencing retention.- 2.2 Theory of separations and retention characteristics.- 2.3 Column efficiency.- 2.4 Band broadening.- 2.5 Resolution.- 2.6 Quantitation in chromatography.- References.- 3 Plane chromatography.- 3.1 Thin-layer chromatography (TLC).- 3.2 Paper chromatography (PC).- 3.3 Electrophoresis.- References.- 4 Liquid phase chromatography on open columns.- 4.1 Practical aspects/considerations.- 4.2 Modes of chromatography.- 4.3 Adsorption chromatography.- 4.4 Partition column chromatography.- 4.5 Ion-exchange chromatography.- 4.6 Salting out chromatography.- 4.7 Inorganic ion-exchangers.- 4.8 Liquid ion-exchangers.- 4.9 Gel ion-exchangers.- 4.10 Gel chromatography.- 4.11 Affinity chromatography.- 4.12 Covalent chromatography.- 4.13 Chromatofocusing.- References.- 5 Gas chromatography (GC).- 5.1 Principles of GC.- 5.2 GC instrumentation.- 5.3 Sampling techniques.- 5.4 The column and column oven.- 5.5 Detectors for GC.- 5.6 Capillary column gas chromatography.- 5.7 Applications of GC.- 5.8 Gas-solid chromatography.- References.- 6 High performance liquid chromatography (HPLC).- 6.1 Modes of chromatography.- 6.2 Overview of HPLC instrumentation.- 6.3 Theory.- 6.4 Detailed discussion of HPLC instrumentation.- 6.5 Column packings and stationary phases for LC.- 6.6 Adsorption chromatography.- 6.7 Liquid-liquid partition chromatography (LLC).- 6.8 Chemically-bonded stationary phases (BP) for HPLC.- 6.9 Chiral stationary phases.- 6.10 Ion-exchange chromatography (IEC).- 6.11 Ion pairing.- 6.12 Size exclusion chromatography.- 6.13 LC method development.- 6.14 Quantitative analysis.- 6.15 Preparative liquid chromatography (PLC).- 6.16 Microcolumns in liquid chromatography.- 6.17 Applications of HPLC.- References.- 7 Spectroscopic techniques and chromatography.- 7.1 Chromatographic requirements.- 7.2 Mass spectroscopy-chromatographic techniques (GC-MS, HPLC-MS).- 7.3 Infrared spectrophotometry.- 7.4 UV-visible spectrophotometry.- 7.5 Atomic spectroscopy.- References.- 8 Processing of chromatographic data.- 8.1 Methods of recording the chromatographic signal.- 8.2 Data collection and processing.- References.- 9 Model experiments in chromatographic techniques.- 9.1 List of experiments.- 9.2 Section A. Paper chromatography.- 9.3 Section B. Electrophoresis and related techniques.- 9.4 Section C. Thin-layer chromatography.- 9.5 Section D. Column chromatography.- 9.6 Section E. Gas chromatography.- 9.7 Section F. High performance liquid chromatography (HPLC).- References.