Chlamydia Trachomatis: Methods and Protocols

ISBN-13: 9781493996964 / Angielski / Miękka / 2020 / 289 str.

Amanda Claire Brown

Recenzje

“Students, postgraduates, scientists and researchers, public health researchers, and microbiologists will find this book of immense interest. … This is an important book with current information on cutting‐edge methods and protocols for Chlamydia trachomatis. Given the rapid advances in this field, this book will appeal to its intended audience.” (Omer Iqbal, Doody’s Book Reviews, January 27, 2020)

Spis treści

1. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA)

            Kevin E. Eboigbodin and Mark Hoser

 

2. Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue

            Naraporn Somboonna and Ilada Choopara

 

3. Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae

            Kevin E. Eboigbodin

 

4. Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay

            Kevin E. Eboigbodin

 

5. Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth

            Tomislav Meštrović, Dezső P. Virok, Sunčanica Ljubin-Sternak, Tímea Raffai, Katalin Burián, and Jasmina Vraneš

 

6. Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples

            Amanda Claire Brown and Mette T. Christiansen

 

7. Multi Locus Sequence Typing (MLST) of Chlamydiales

            Martina Jelocnik, Adam Polkinghorne, and Yvonne Pannekoek

 

8. Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method

            Deborah C. Holt, Patiyan Andersson, Cameron Buckley, David M. Whiley, and Phillip M. Giffard

 

9. Dual RNA-Seq of Chlamydia and Host Cells

            James W. Marsh, Regan Hayward, Amol Shetty, Anup Mahurkar, Michael S. Humphrys, and Garry S.A. Myers

 

10. Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection

            Oleg V. Podgorny, Nadezhda F. Polina, and Vassili N. Lazarev

 

11. Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions

            Katerina Wolf, Mostafa Rahnama, and Kenneth A. Fields

 

12. Mutagenesis of Chlamydia trachomatis Using TargeTron

            Mary M. Weber and Robert Faris

 

13. Forward and Reverse Genetic Analysis of Chlamydia

            Mateusz Kędzior and Robert J. Bastidas

 

14. A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions

            Rachel Ende and Isabelle Derré

 

15. Rodent Infections for Chlamydia spp.

            Charles W. Armitage, Alison J. Carey, and Kenneth W. Beagley

 

16. Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy

            Steven Liang and James B. Mahony

 

17. Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane

            Macy G. Olson, Lisa M. Jorgenson, Ray E. Widner, and Elizabeth A. Rucks

 

18. High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis

            Amanda Claire Brown and Nicole L. Kushner

Blurb

This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.