Chimera Research: Methods and Protocols

ISBN-13: 9781493995264 / Angielski / Miękka / 2020 / 234 str.

Insoo Hyun;Alejandro De Los Angeles

Opis

This volume addresses challenging new questions surrounding stem cell-based chimera research. This book is organized into three parts: Part One provides readers with a summary of different human donor cell types. The chapters in this section discuss ways to evaluate new types of pluripotent stem cells; the derivation of naive and primed pluripotent stem cells from mouse preimplantation embryos; and the ethical and regulatory complexities of informed consent for the procurement of somatic cells. Part Two discusses methods for generating chimeras. The chapters here look at chick models and human-chick organizer grafts; generating human-pig interspecies chimeras; and techniques for transplanting mouse neural stem cells into a mouse disease model for stroke. Part Three concludes the book with a look at ongoing ethical controversies and new scientific directions. Chapters in this part cover the ethics of crossing the xenobarrier; animal welfare; experimentation with spermatogonial stem cells; and cautious approaches to human-monkey chimera studies to further understand complex human brain disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Chimera Research: Methods and Protocols is a valuable resource for scientists interesting in using chimeras as a research tool while also taking into consideration their complex ethical scopes.

Spis treści

Preface…

Table of Contents…

Contributing Authors…

Part I Human Donor Cell Types: Potency and Position 

1. Frontiers of Pluripotency

Alejandro De Los Angeles

2. Highly Efficient Derivation of Pluripotent Stem Cells from Mouse Preimplantation and Postimplantation Embryos in Serum-Free Conditions 

Alejandro De Los Angeles, Daiji Okamura, and Jun Wu

3. Derivation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells

Ryohichi Sugimura

4. Cancer Stem Cells: Concepts, Challenges and Opportunities for Cancer Therapy

May Yin Lee, Rajshekhar R. Giraddi, and Wai Leong Tam

5. Informed Consent Issues for Cell Donors

Insoo Hyun

Part II Non-Human Hosts: Species and Developmental Stages 

 

6. Chick Models and Human-Chick Organizer Grafts

Iain Martyn, Tatiane Y. Kanno, and Ali H. Brivanlou

 

7. The Engraftment of Lentiviral Vector-Transduced Human CD34+ Cells into Humanized Mice

Yoon-Sang Kim, Matthew Wielgosz, and Byoung Ryu

 

8. Pig Chimeric Model with Human Pluripotent Stem Cells 

Cuiqing Zhong, Jun Wu, and Juan Carlos Izpisua Belmonte

9. Embryonic Chimeras with Human Pluripotent Stem Cells

Alejandro De Los Angeles, Masahiro Sakurai, and Jun Wu

 

10. Neural Stem Cell Transplantation into a Mouse Model of Stroke 

Alejandro De Los Angeles

11. Ethical Standards for Chimera Research Oversight

Insoo Hyun

 

Part III New Directions and Controversies

12. Ethical Considerations in Crossing the Xenobarrier

Sebastian Porsdam Mann, Rosa Sun, and Göran Hermerén

13. Neurological Chimeras and the Moral Staircase

Daniel Counihan

14. Isolation, Cryopreservation, and Transplantation of Spermatogonial Stem Cells 

Nilam Sinha, Eoin C. Whelan, and Ralph L. Brinster

15. Human-Monkey Chimeras for Modeling Human Disease: Opportunities and Challenges

Alejandro De Los Angeles, Insoo Hyun, Stephen R. Latham, John D. Elsworth, and D. Eugene Redmond Jr.

Blurb

This volume addresses challenging new questions surrounding stem cell-based chimera research. This book is organized into three parts: Part One provides readers with a summary of different human donor cell types. The chapters in this section discuss ways to evaluate new types of pluripotent stem cells; the derivation of naïve and primed pluripotent stem cells from mouse preimplantation embryos; and the ethical and regulatory complexities of informed consent for the procurement of somatic cells. Part Two discusses methods for generating chimeras. The chapters here look at chick models and human-chick organizer grafts; generating human-pig interspecies chimeras; and techniques for transplanting mouse neural stem cells into a mouse disease model for stroke. Part Three concludes the book with a look at ongoing ethical controversies and new scientific directions. Chapters in this part cover the ethics of crossing the xenobarrier; animal welfare; experimentation with spermatogonial stem cells; and cautious approaches to human-monkey chimera studies to further understand complex human brain disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chimera Research: Methods and Protocols is a valuable resource for scientists interesting in using chimeras as a research tool while also taking into consideration their complex ethical scopes.