Chemotaxis: Methods and Protocols

ISBN-13: 9781617796425 / Angielski / Miękka / 2012 / 538 str.

Jin, Tian

Opis

Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology. In Chemotaxis: Methods and Protocols, expert researchers in the field provide state-of-the-art methods for investigating cell migration behaviors, studying molecular components involved in detecting extracellular signals and directing cell movement, visualizing spatiotemporal dynamics of the components in signaling networks of chemotaxis in real time, and constructing quantitative models that simulate chemoattractant-induced cell responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.§Comprehensive and cutting-edge, Chemotaxis: Methods and Protocols serves scientists with practical guidance on the diverse methodologies that are currently propelling chemotaxis research forward in order to further our understanding of this vital biological system.

Recenzje

From the reviews: "The purpose is to provide an overview of and practical guidance on the diverse methodologies involved in chemotaxis research. ... Each chapter includes an introduction to the topic, lists of necessary materials and reagents, laboratory protocol, and notes on troubleshooting and avoiding pitfalls, followed by current references. ... This will serve as a valuable resource for students and scientists seeking practical guidance on diverse methodologies in chemotaxis research." (Omer Iqbal, Doody's Review Service, April, 2010)

Spis treści

1. Microfluidic Techniques for the Analysis of Bacterial Chemotaxis Derek L. Englert, Arul Jayaraman, and Michael D. Manson 2. Prokaryotic Phototaxis Wouter D. Hoff, Michael A. van der Horst, Clara B. Nudel, and Klaas J. Hellingwerf 3. Photoorientation in Photosynthetic Flagellates Donat-Peter Häder and Michael Lebert 4. Dictyostelium Slug Phototaxis Sarah J. Annesley and Paul R. Fisher 5. Electrotaxis and Wound Healing: Experimental Methods to Study Electric Fields as a Directional Signal for Cell Migration Guangping Tai, Brian Reid, Lin Cao, and Min Zhao 6. Chemotropism During Yeast Mating Peter J. Follette and Robert A. Arkowitz 7. Group Migration and Signal Relay In Dictyostelium Paul W. Kriebel and Carole A. Parent 8. Quantitative Analysis of Distal Tip Cell Migration in C. elegans Myeongwoo Lee and Erin J. Cram 9. Inflammation and Wound Healing in Drosophila Brian Stramer and Will Wood 10. Neutrophil Motility In Vivo Using Zebrafish Jonathan R. Mathias, Kevin B. Walters, and Anna Huttenlocher 11. Chemotaxis in Neutrophil-like HL-60 Cells Arthur Millius and Orion D. Weiner 12. Chemokine Receptor Dimerization and Chemotaxis José Miguel Rodríguez-Frade, Laura Martinez Muñoz, Borja L. Holgado, and Mario Mellado 13. Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes Chung Park, Il-Young Hwang, and John H. Kehrl 14. Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM Microscopy Xuehua Xu, Peter Johnson, and Susette C. Mueller 15. In vivo Assay for Tumor Cell Invasion Lorena Hernandez, Tatiana Smirnova, Jeffrey Wyckoff, John Condeelis, and Jeffrey E. Segall 16. Quantitative Studiesof Neuronal Chemotaxis in 3D William J. Rosoff, Ryan G. McAllister, Geoffrey J. Goodhill, and Jeffrey S. Urbach 17. Assays for Chemotaxis and Chemoattractant-Stimulated TorC2 Activation and PKB Substrate Phosphorylation in Dictyostelium Yoichiro Kamimura, Ming Tang, and Peter Devreotes 18. Biochemical Responses to Chemoattractants in Dictyostelium: Ligand-Receptor Interactions and Downstream Kinase Activation Xin-Hua Liao and Alan R. Kimmel 19. Quantifying In Vivo Phosphoinositide Turnover in Chemotactically-Competent Dictyostelium Cells Nadine Pawolleck and Robin S.B. Williams 20. In Vivo Measurements of Cytosolic Calcium in Dictyostelium discoideum Claire Y. Allan and Paul R. Fisher 21. Chemokine Receptor Signaling and HIV Infection Yuntao Wu 22. Spatiotemporal Stimulation of Single Cells Using Flow Photolysis Carsten Beta 23. Spatio-Temporal Regulation of Ras-GTPases During Chemotaxis Atsuo T. Sasaki and Richard A. Firtel 24. FRAP Analysis of Chemosensory Components of Dictyostelium Carrie A. Elzie and Chris Janetopoulos 25. Monitoring Dynamic GPCR Signaling Events Using Fluorescence Microscopy, FRET Imaging and Single Molecule Imaging Xuehua Xu, Joseph A. Brzostowski, and Tian Jin 26. Imaging Actin Cytoskeleton Dynamics in Dictyostelium Chemotaxis Günther Gerisch 27. Analysis of Actin Assembly by In Vitro TIRF Microscopy Dennis Breitsprecher, Antje K. Kiesewetter, Joern Linkner, and Jan Faix 28. Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living Dictyostelium Cells Yukihiro Miyanaga, Satomi Matsuoka, and Masahiro Ueda 29. Imaging B-cell Receptor Signaling by Single Molecule Techniques Pavel Tolar and Tobias