Chemotaxis: Methods and Protocols

ISBN-13: 9781493980598 / Angielski / Miękka / 2018 / 425 str.

Tian Jin; Dale Hereld

Spis treści

1.      In Situ Assays of Chemotropism during Yeast Mating
David E. Stone and Robert A. Arkowitz

2.     Imaging Polarization in Budding Yeast
Allison W. McClure, Chi-Fang Wu, Sam A. Johnson, and Daniel J. Lew

3.     Migration of Dictyostelium discoideum to the Chemoattractant Folic Acid
Karl J. Aufderheide and Chris Janetopoulos

4.     Mitochondrial Stress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideum Cells
Sui Lay, Oana Sanislav, Sarah J. Annesley, and Paul R. Fisher

5.     Studying Chemoattractant Signal Transduction Dynamics in Dictyostelium by BRET
A.F.M. Tariqul Islam, Branden M. Stepanski, and Pascale G. Charest

6.     Wave Patterns in Cell Membrane and Actin Cortex Uncoupled from Chemotactic Signals
Günther Gerisch and Mary Ecke

7.     Chemotactic Blebbing in Dictyostelium Cells
Evgeny Zatulovskiy and Robert R. Kay

8.     Dissecting Spatial and Temporal Sensing in Dictyostelium Chemotaxis Using a Wave Gradient Generator
Akihiko Nakajima and Satoshi Sawai

9.     Employing Dictyostelium as an Advantageous 3Rs Model for Pharmacogenetic Research
Grant P. Otto, Marco Cocorocchio, Laura Munoz, Richard A. Tyson, Till Bretschneider, and Robin S.B. Williams

10.   Identification of Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis
Xiumei Cao and Jianshe Yan

11.   Biochemical Responses to Chemically Distinct Chemoattractants during the Growth and Development of Dictyostelium
Netra Pal Meena and Alan R. Kimmel

12.   Live Imaging of Border Cell Migration in Drosophila
Wei Dai and Denise J. Montell

13.   ShRNA-Induced Gene Knock-Down In Vivo to Investigate Neutrophil Function
Abdul Basit, Wenwen Tang, and Dianqing Wu

14.   Studying Neutrophil Migration In Vivo Using Adoptive Cell Transfer
Yoshishige Miyabe, Nancy D. Kim, Chie Miyabe, and Andrew D. Luster

15.   Intravital Two-Photon Imaging of Lymphocytes Crossing High Endothelial Venules and Cortical Lymphatics in the Inguinal Lymph Node
Chung Park, Il-Young Hwang, and John H. Kehrl

16.   Flow Cytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response
Yuntao Wu

17.    Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D
Olivia Susanto, Andrew J. Muinonen-Martin, Max Nobis, and Robert H. Insall

18.    4D Tumorigenesis Model for Quantitating Coalescence, Directed Cell Motility and Chemotaxis, Identifying Unique Cell Behaviors and Testing Anti-Cancer Drugs
Spencer Kuhl, Edward Voss, Amanda Scherer, Daniel F. Lusche, Deborah Wessels, and David R. Soll

19.    An Experimental Model for Simultaneous Study of Migration of Cell Fragments, Single Cells and Cell Sheets
Yao-Hui Sun, Yuxin Sun, Kan Zhu, Bruce W. Draper, Alex Mogilner, and Min Zhao

20.    Axon Guidance Studies Using a Microfluidics-Based Chemotropic Gradient Generator
Zac Pujic, Huyen Nguyen, Nick Glass, Justin Cooper-White, and Geoffrey J. Goodhill

21.    Visualization of Actin Assembly and Filament Turnover by In Vitro Multi-Color TIRF Microscopy
Moritz Winterhoff, Stefan Brühmann, Christof Franke, Dennis Breitsprecher, and Jan Faix

22.    Quantitative Monitoring Spatiotemporal Activation of Ras and PKD1 Using Confocal Fluorescent Microscopy
Xuehua Xu, Michelle Yun, Xi Wen, Joseph Brzostowski, Wei Quan, Q. Jane Wang, and Tian Jin

23.    Fluorescence Readout of a Patch Clamped Membrane by Laser Scanning Microscopy
Matthias Gerhardt, Michael Walz, and Carsten Beta

24.    Use of Resonance Energy Transfer Techniques for In Vivo Detection of Chemokine Receptor Oligomerization
Laura Martínez-Muñoz, José Miguel Rodríguez-Frade, and Mario Mellado

25.    Multi-State Transition Kinetics of Intracellular Signaling Molecules by Single-Molecule Imaging Analysis
Satomi Matsuoka, Yukihiro Miyanaga, and Masahiro Ueda

26.    Mathematics of Experimentally Generated Chemoattractant Gradients
Marten Postma and Peter J.M. van Haastert

27.    Modeling Excitable Dynamics of Chemotactic Networks
Sayak Bhattacharya and Pablo A. Iglesias

Blurb

This second edition expands on the first edition with new chapters describing methods for studying cell movement, molecular components involved in chemotaxis, spatiotemporal dynamics of signaling components, and quantitative modeling, as well as several updated chapters from the first edition. Various methods to investigate directional cell growth and movements are presented in Chapters 1-20. These chapters contains experimental procedures to visualize and measure migration behaviors of different kinds of organisms, including chemotropism in the budding yeast; cell growth and migration of D. discoideum; border cell migration in Drosophila; chemotaxis of mouse and human neutrophils; and HIV-induced T cell chemotactic response.Chemotaxis: Methods and Protocols, Second Edition also contains microscopy procedures for studying breast cancer cell migration, tumor cell invasion in vivo, and axon guidance. The book concludes with Chapters 21-27 describing methods that measure spatiotemporal dynamics of signaling components involved in chemotaxis; introduce imaging techniques, such as TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models of experimentally generated chemoattractant gradients. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Chemotaxis: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the diverse methodologies that are propelling chemotaxis research forward.