Chemokine Protocols

ISBN-13: 9781617371516 / Angielski / Miękka / 2010 / 353 str.

Amanda E. I. Proudfoot; Timothy N. C. Wells; Christine Power

Opis

The chemokines family of small proteins are involved in numerous b- logical processes ranging from hematopoiesis, angiogenesis, and basal l- kocyte trafficking to the extravasation and tissue infiltration of leukocytes in response to inflammatory agents, tissue damage, and bacterial or viral infection. Chemokines exert their effects through a family of seven G-protein coupled transmembrane receptors. Worldwide interest in the chemokine field surged dramatically early in 1996, with the finding that certain chemokine receptors were the elusive coreceptors, required along with CD4, for HIV infection. Today, though over 40 human chemokines have been described, the n- ber of chemokine receptors lags behind--only 17 human chemokine receptors have been identified so far. What has emerged over the years is that most chemokine receptors bind several distinct ligands, and indeed the majority of chemokines are able to bind to multiple chemokine receptors, explaining to some extent the apparent disparity in the numbers of chemokines and rec- tors. Yet in spite of the apparent redundancy in chemokine/chemokine rec- tor interactions, it is clear that in vivo, spatial, temporal, and indeed cell- and tissue-specific expression of both chemokines and their receptors are imp- tant factors in determining the precise nature of cellular infiltrates in phy- ological and pathological processes.

Recenzje

highly recommended...Seldom does on find so much valuable information, concisely presented and with such obvious potential for the future, made available in one volume." - E-Streams

"...Chemokine Protocols is a collection of clear, well written protocols and I would recommend it as an excellent starting point for any scientist with an interest in chemokines." - Molecular Biotechnology

Spis treści

Purification of Chemokines from Natural Sources, Jens-M. Schröder. Cloning of Novel Chemokines Using a Signal Sequence Trap Method, Toshio Imai. Chemokine Expression in Insect Cells, Toshio Imai. Expression of Chemokines in Escherichia coli, Michael D. Edgerton, Lars-Ole Gerlach, Thomas P. Boesen, and Bernard Allet. Expression of Chemokines in the Periplasmic Space of E. coli, Jochen Pfirstinger and Matthias Mack. Synthesis of Chemokines, Ian Clark-Lewis. Identification of Novel Chemokines from Expressed Sequence Tag Databases, Timothy N. C. Wells and Manuel C. Peitsch. Purification of Recombinant Chemokines from E. coli, Amanda E. I. Proudfoot and Frédéric Borlat. Chemokine Receptor Cloning, Philip M. Murphy. Generation of Stable Cell Lines Expressing Chemokine Receptors, Christine A. Power and Alexandra Meyer. Modified Microchemotaxis Assays, Dennis D. Taub. Transwell Chemotaxis, Paul D. Ponath, Juan Wang, and Heidi Heath. Endothelial Cell Chemotaxis Assays, Darcey Black. Radiolabeled Chemokine Binding Assays, Bruce L. Daugherty, Salvatore J. Siciliano, and Martin S. Springer. Scintillation Proximity Binding Assay, Sami Alouani. Calcium Mobilization, Raphaële Buser and Amanda E. I. Proudfoot. Actin Polymerization, Jörn Elsner and Alexander Kapp. Reactive Oxygen Release, Jörn Elsner and Alexander Kapp. Histamine Release, Michael A. Lett-Brown and Rafeul Alam. Measurement of Phosphoinositide 3-Kinase Activity, Stephen G. Ward. Glycosaminoglycan Binding Assays, Arlene J. Hoogewerf and Gabriele S. V. Kuschert. CFU-A Assay for Measurement of the Antiproliferative Effects of Chemokines on Murine Early Hemopoietic Progenitors, Gerard J. Graham and Mary G. Freshney. Downmodulation and Recycling of Chemokine Receptors, Matthias Mack and Detlef Schlöndorff. Analysis of Chemokine Receptor Endocytosis and Recycling, Nathalie Signoret and Mark Marsh. Chemokine Inhibition of HIV Infection, Jacqueline D. Reeves and Graham Simmons. The Production of Chemokine SpecificMonoclonal Antibodies: Chemokine RANTES, Peter J. Nelson. Monoclonal Antibodies to Chemokine Receptors, Paul D. Ponath, Nassim Kassam and Shixin Qin. Targeted Expression of Chemokines In Vivo, Iqbal Grewal, Long Gu, Susan Tseng, and Barrett J. Rollins. Chemokine Knockout Mice, Marc E. Rothenberg. Chemokine Receptor Knockout Mice, Ji-Liang Gao and Philip M. Murphy. Measurement of Eosinophil Accumulation In Vivo, Maria-Jesus Sanz, Peter J. Jose, and Timothy J. Williams. Murine Model of Allergic Lung Inflammation, Sami Alouani, Pierre Juillard, and Yolande Chvatchko. Murine Models of Airway Inflammation, Emma M. Campbell and Nicholas W. Lukacs. Rat Models of Respiratory Inflammation, Andrew S. McWilliam and Patrick G. Holt. Murine Model of Crescentic Nephritis, Clare Lloyd and Jose-Carlos Gutierrez-Ramos. Rabbit Models of Pneumonia, Peritoneal Sepsis, and Lung Injury, Charles W. Frevert, Gustavo Matute-Bello, and Thomas R. Martin. Index.

Blurb

In the past decade research has established the biological importance of chemokines: they play a major role in leukocyte trafficking, in the recruitment of leukocytes to inflammatory sites, and are coreceptors along with CD4 for HIV cell entry. In Chemokine Protocols, expert investigators describe in detail important techniques usedchemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo. Each method also includes relevant background information, as well as providing a useful bibliography that renders the study of chemokines accessible at all levels of experience.
Comprehensive and highly practical, Chemokine Protocols offers experimental and clinical chemokine researchers today's gold-standard collection of proven methods for analyzing this biologically ubiquitous and important class of proteins.