Chemical and Synthetic Approaches in Membrane Biology

ISBN-13: 9781493968350 / Angielski / Twarda / 2017 / 270 str.

Arun K. Shukla

Opis

This practical volume brings together a broad range of topics related to membrane biology research with particular emphasis on novel approaches, technology platforms, and emerging tools in this area of study.

Spis treści

1. Lipid-Coated Gold Nanoparticles as Probes for Membrane Binding

            Desmond J. Hamilton, Yuheng Cai, Rupinder Kaur, Grant W. Marquart, Marilyn R. Mackiewicz, and Scott M. Reed

 

2. Delving into Lipid-Driven Endocytic Mechanisms Using Biomimetic Membranes

            Josef Madl, Sarah Villringer, and Winfried Römer

 

3. Lipid Bilayer Patterns Fabrication by One-Photon Lithography

            M. Florencia Sánchez and Dolores C. Carrer

 

4. Approaches for Preparation and Biophysical Characterization of Transmembrane β-Barrels

            Bharat Ramasubramanian Iyer, Ankit Gupta, and Radhakrishnan Mahalakshmi

 

5. Probing the Dynamics of Plasma Membrane Glutamate Transporters in Real-Time by Total Internal Fluorescence Reflection Microscopy

            Eliana S. Di Cairano, Paola Marciani, Stefania Moretti, and Carla Perego

 

6. Fluorescence-Based Screening of Membrane Proteins for Structural Studies

            Jyoti Kumari, Surbhi Dhingra, and Janesh Kumar

 

7. Single-Molecule Localization Super-Resolution Microscopy of Synaptic Proteins

            Francisco J. Barrantes

 

8. Chemical Methods for Monitoring Protein Fatty-Acylation

            Xiaoqiu Yuan and Howard C. Hang

 

9. Quantum Dot Toolbox in Membrane Neurotransmitter Transporter Research

            Lucas B. Thal, Danielle M. Bailey, Oleg Kovtun, and Sandra J. Rosenthal

 

10. Generation of Synthetic Antibody Fragments to Detergent Solubilized Membrane Proteins

            Serdar Uysal and Anthony Kossiakoff

 

11. Pulse-Chase Covalent Labeling Technique for Monitoring GPCR Endocytosis

            Hidetoshi Kumagai and Yuichi Ikeda

 

12. Sample Preparation of Rhodopsins in the E. coli Membrane for In Situ Magic Angle Spinning Solid-State Nuclear Magnetic Resonance Studies

            Meaghan E. Ward, Vladimir Ladizhansky, and Leonid S. Brown

Blurb

This practical volume brings together a broad range of topics related to membrane biology research with particular emphasis on novel approaches, technology platforms, and emerging tools in this area of study. Beginning with chapters pertaining to artificial or designer membrane mimetics that can be utilized for in vitro studies of membrane-lipid interactions, it then continues by covering the approaches and methodologies to directly investigate membrane protein structure, localization, and dynamics.  Written for the Springer Protocols Handbooks series, this collection focuses on in-depth, hands-on protocols from experts in the field. 

Authoritative and cutting-edge, Chemical and Synthetic Approaches in Membrane Biology serves as an ideal guide to researchers investigating the vital properties of cellular membranes.