Chemical Genomics

ISBN-13: 9780521889483 / Angielski / Twarda / 2012 / 344 str.

Haian Fu

Opis

Advances in chemistry, biology, and genomics coupled with laboratory automation and computational technologies have led to the rapid emergence of the multidisciplinary field of chemical genomics. This edited text with contributions from experts in the field discusses evolving concepts, essential techniques, and a wide range of applications to help further the study of chemical genomics. The beginning chapters provide an overview of the basic principles of chemical biology and chemical genomics. This is followed by a technical section that describes the sources of small molecule chemicals, the basics of high-throughput screening technologies, various bioassays for biochemical-, cellular-, and organism-based screens, and computational approaches. The final chapters connect the chemical genomics field with personalized medicine and the druggable genome for future discovery of new therapeutics. In selected chapters, a resource section is included for further information. This book provides an introduction to the principles of chemical genomics, serves as a resource for the associated technologies, and gives readers general concepts, useful vocabulary, practical techniques, and broad, forward-thinking perspectives. This book will be valuable to researchers, professionals, and students in many fields besides chemical genomics, including biology, biomedicine, chemistry, and drug discovery.

Recenzje

'Author Haian Fu has effectively assembled his textbook, Chemical Genomics, in a manner that is easy to comprehend and builds a basic understanding of chemical genomics.' Danielle Gerhard, Yale Journal of Biology and Medicine

Spis treści

Part I. Overviews: 1. Harnessing the power of chemistry for biology and medicine Cheryl L. Meyerkord and Haian Fu; 2. Chemical genomics: exploring biology with small organic molecules Nicholas Aberle and Craig M. Crews; 3. Chemical proteomics Akihisa Matsuyama, Yoko Yashiroda and Minoru Yoshida; Part II. Molecules for Chemical Genomics: 4. Diversity-oriented synthesis Warren R. J. D. Galloway, Richard J. Spandl, Andreas Bender, Gemma L. Thomas, Monica Diaz-Gavilan, Kieron M. G. O'Connell and David R. Spring; 5. Targeted chemical libraries Gregory P. Tochtrop and Ryan E. Looper; 6. Fragment-based ligand discovery Sandra Bartoli, Antonella Squarcia and Daniela Fattori; 7. Basics and principles for building natural product-based libraries for HTS Ronald J. Quinn; Part III. Basics of High Throughput Screening: 8. Essentials for high throughput screening operations Stewart P. Rudnicki, James V. Follen, Nicola J. Tolliday and Caroline E. Shamu; 9. High content analysis and screening: basics, instrumentation and applications Paul A. Johnston; 10. Phenotypic screens with model organisms Grant N. Wheeler, Robert A. Field and Matthew L. Tomlinson; 11. Screening informatics and ChemInformatics Lestyn Lewis; Part IV. Chemical Genomics Assays and Screens: 12. Basics on HTS assay design and optimization Eduard Sergienko; 13. Molecular sensors for transcriptional and post-transcriptional assays Douglas S. Auld and Natasha Thorne; 14. Fluorescence-based platforms for HTS assays – FP and TR-FRET Yuhong Du and Jonathan Havel; 15. Compound profiling with high content screening methodology Thomas Mayer and Stephan Schürer; 16. Use of transgenic zebrafish in a phenotypic screen for angiogenesis inhibitors Jaeki Min, Yuhong Du, Brenda Bondesen, Brian Revennaugh, Peter Eimon and Ray Dingledine; 17. Flow cytometry multiplexed screening methodologies Virginia M. Salas, J. Jacob Strouse, Zurab Surviladze, Irena Ivnitski-Steele, Bruce S. Edwards and Larry A. Sklar; 18. Basic principles and practices of computer-aided screening and drug design Chao-Yie Yang, Denzil Bernard and Shaomeng Wang; 19. Computational approach for drug target identification Honglin Li, Mingyue Zheng, Xiaofeng Liu and Hualiang Jiang; 20. Label-free biosensor technologies for assay development and HTS Yuhong Du and Arron Xu; Part V. Chemical Genomics and Medicine: 21. Pharmacogenomics to link genetic background with therapeutic efficacy and safety Mark M. Bouzyk, Weining Tang and Brian Leyland-Jones; 22. Drugs, genomic response signatures, and customized cancer therapy Rafael Rosell, Teresa Moran and Miguel Taron; Appendix. Current drug targets and the druggable genome Andreas Russ.