Characterization of Nanoparticles Intended for Drug Delivery

ISBN-13: 9781493984602 / Angielski / Miękka / 2018 / 256 str.

Scott E. McNeil

Spis treści

Preface

Table of Contents…

Contributing Authors…

Part I Introduction

1. Evaluating Nanomedicines: Obstacles and Advancements

Magdalena Swierczewska, Rachael M. Crist, and Scott E. McNeil

Part II Sterility and Endotoxin Testing

2. Detection of Bacterial Contamination in Nanoparticle Formulations by Agar Plate Test

Timothy M. Potter, Barry W. Neun, Anna N. Ilinskaya, and Marina A. Dobrovolskaia

3. Considerations and Some Practical Solutions to Overcome Nanoparticle Interference with LAL Assays and to Avoid Endotoxin Contamination in Nanoformulations

Barry W. Neun and Marina A. Dobrovolskaia

Part III Physicochemical Characterization

4. Elemental Analysis in Biological Matrices Using ICP-MS

Matthew N. Hansen and Jeffrey D. Clogston

5. PEG Quantitation Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection

Mackensie C. Smith and Jeffrey D. Clogston

6. Quantitation of Surface Coating on Nanoparticles Using Thermogravimetric Analysis

Alpana A. Dongargaonkar and Jeffrey D. Clogston

7. Immunoelectron Microscopy for Visualization of Nanoparticles

Sarah R. Anderson, David Parmiter, Ulrich Baxa, and Kunio Nagashima 

8. Imaging of Liposomes by Transmission Electron Microscopy

Ulrich Baxa

Part IV Immunology

9. Updated Method for In Vitro Analysis of Nanoparticle Hemolytic Properties

Barry W. Neun, Anna N. Ilinskaya, and Marina A. Dobrovolskaia

10. In Vitro Assessment of Nanoparticle Effects on Blood Coagulation

Timothy M. Potter, Jamie C. Rodriguez, Barry W. Neun, Anna N. Ilinskaya, Edward Cedrone, and Marina A. Dobrovolskaia

11. In Vitro Analysis of Nanoparticle Effects on the Zymosan Uptake by Phagocytic Cells

Timothy M. Potter, Sarah L. Skoczen, Jamie C. Rodriguez, Barry W. Neun, Anna N. Ilinskaya, Edward Cedrone, and Marina A. Dobrovolskaia

12. Assessing NLRP3 Inflammasome Activation by Nanoparticles

Bhawna Sharma, Christopher B. McLeland, Timothy M. Potter, Stephan T. Stern, and Pavan P. Adiseshaiah

13. Analysis of Complement Activation by Nanoparticles

Barry W. Neun, Anna A. Ilinskaya, and Marina A. Dobrovolskaia 

14. Methods for Analysis of Nanoparticle Immunosuppressive Properties In Vitro and In Vivo

Timothy M. Potter, Barry W. Neun, and Marina A. Dobrovolskaia

15. Analysis of Pro-Inflammatory Cytokine and Type II Interferon Induction by Nanoparticles

Timothy M. Potter, Barry W. Neun, Jamie C. Rodriguez, Anna N. Ilinskaya, and Marina A. Dobrovolskaia 

16. Analysis of Nanoparticle Adjuvant Properties In Vivo

Barry W. Neun and Marina A. Dobrovolskaia

17. In Vitro and In Vivo Methods for Analysis of Nanoparticle Potential to Induce Delayed Type Hypersensitivity Reactions

Timothy M. Potter, Barry W. Neun, and Marina A. Dobrovolskaia

18. Autophagy Monitoring Assay II: Imaging Autophagy Induction in LLC-PK1 Cells Using GFP-LC3 Protein Fusion Construct

Pavan P. Adiseshaiah, Sarah L. Skoczen, Jamie C. Rodriguez, Timothy M. Potter, Krishna Kota, and Stephan T. Stern

Part V Drug Release and In Vivo Efficacy

19. Improved Ultrafiltration Method to Measure Drug Release from Nanomedicines Utilizing a Stable Isotope Tracer

Sarah L. Skoczen and Stephan T. Stern

20. Designing an In Vivo Efficacy Study of Nanomedicines for Preclinical Tumor Growth Inhibition

Pavan P. Adiseshaiah and Stephan T. Stern

Blurb

This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents. The chapters in this book are divided into 5 parts and cover topics such as: advances and obstacles in nanomedicine research; methods to test sterility and endotoxin, physicochemical features, immunological effects, drug release, and in vivo efficacy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Characterization of Nanoparticles Intended for Drug Delivery, Second Edition is a valuable tool for researchers and pharmaceutical and biotechnology developers who are evaluating the clinical potential of nanomedicines in preclinical studies.>