Characterization of Interleukin-33 and the IL-33 Receptor Complex

ISBN-13: 9783639148183 / Angielski / Miękka / 2010 / 136 str.

Shafaqat Ali

Opis

Two pivotal aspects of the IL-33 system are characterized in this study: First the composition and function of the IL-33 receptor complex on IL-33 responder cells and second the generation of biologically active IL-33 by the IL-33 producer cell. IL-33 binds to the IL-33 receptor -chain (ST2) and as is shown here, this results in the recruitment of interleukin-1 receptor accessory protein (IL-1RAcP) as the co-receptor. The IL-33-dependent interaction of membrane bound and soluble form of IL-1RAcP and IL-33R -chain was demonstrated in co-immunoprecipitation assays. Common notion in the field is that IL-33, like IL-1 and IL-18, requires processing by caspase-1 to a mature form, in order to achieve biological activity as a cytokine. Contrary to the current dogma, here it is described that IL-33 is biologically active as unprocessesed full length molecule. Full length IL-33 binds to the IL-33 receptor- chain and mediates the recruitment of IL-1RAcP to activate cells. IL-33 is processed in cells, however not by caspase 1 to a mature, biologically active, IL-33 but instead it is cleaved by caspase 3 at aa175 to yield two products which are both unable to bind to the IL-33 receptor."

O autorze

born in Sialkot (Pakistan) in 1977. He did M.Sc. in Biochemistry (2000), M.Phil. in Biotechnology (2003) and got Promotion to Dr. rer.nat.(in 2009). Presently working as Postdoc. Fellow, in Institute of Immunology, Justus-Liebig-Universität, Gießen Germany. Previously has been working as Research Fellow (2003-06)in NIBGE, Faisalabad, Pakistan.