Cervical Cancer: Methods and Protocols

ISBN-13: 9781493920129 / Angielski / Twarda / 2014 / 413 str.

Daniel Keppler; Athena W. Lin

Opis

Representing the most relevant procedures and technologies aiding the advance of the field of HPV-mediated carcinogenesis of the cervix and other anatomical regions of squamocolumnar transition, such as the anorectum, penis, and oropharynx, Cervical Cancer: Methods and Protocols compiles a detailed collection of practical chapters. The first half of the book covers HPV types, pathogenesis of cervical cancer (CxCA), prevention, and novel potential drug targets, while the second half explores pathology, genomics, modeling of CxCA, and experimental therapeutic strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and vital, Cervical Cancer: Methods and Protocols serves as a valuable resource to both bench scientists and clinicians who step into the realm of high-risk HPVs and CxCA for the first time or those who wish to learn novel approaches or expand their toolbox for the study of CxCA.

Spis treści

Part I: Approaches to the Detection and Analysis of HPV Types

 

1. Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with Cervical Cancer

            Zigui Chen, Luciana Bueno de Freitas, and Robert D. Burk

 

2. A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for the Detection and Typing of Human Papillomavirus

            M. Isabel Micalessi, Gaelle A. Boulet, and J. Jean-Paul Bogers

 

Part II: Molecular Pathogenesis of CxCA

 

3. Replication of Human Papillomavirus in Culture

            Eric J. Ryndock, Jennifer Biryukov, and Craig Meyers

 

4. HPV Binding Assay to Laminin-332/Integrin α6β4 on Human Keratinocytes

            Sarah A. Brendle and Neil D. Christensen

 

5. Methods to Assess the Nucleocytoplasmic Shuttling of the HPV E1 Helicase and Its Effects on Cellular Proliferation and Induction of a DNA Damage Response

            Michaël Lehoux, Amélie Fradet-Turcotte, and Jacques Archambault

 

6. Genetic Methods for Studying the Role of Viral Oncogenes in the HPV Life Cycle

            Jason M. Bodily

 

7. Robust HPV-18 Production in Organotypic Cultures of Primary Human Keratinocytes

            Hsu-Kun Wang, Thomas R. Broker, and Louise T. Chow

 

8. A High-Throughput Cellular Assay to Quantify the p53-Degradation Activity of E6 from Different Human Papillomavirus Types

            David Gagnon and Jacques Archambault

 

9. Retroviral Expression of Human Cystatin Genes in HeLa Cells

            Crystal M. Diep, Gagandeep Kaur, Daniel Keppler, and Athena W. Lin

 

Part III: Vaccinations and Immune Responses to HPV Infections

 

10. Molecular Analysis of Human Papillomavirus Virus-Like Particle Activated Langerhans Cells In Vitro

            Andrew W. Woodham, Adam B. Raff, Diane M. Da Silva, and W. Martin Kast

 

Part IV: Identification of Novel Potential Drug Targets

 

11. Selective Silencing of Gene Target Expression By siRNA Expression Plasmids in Human Cervical Cancer Cells

            Peralta-Zaragoza Oscar, De la O-Gómez Faustino, Deas Jessica, Fernández-Tilapa Gloria, Fierros-Zárate Geny del Socorro, Gómez-Cerón Claudia, Burguete-García Ana, Torres-Poveda Kirvis, Bermúdez-Morales Victor Hugo, Rodríguez-Dorantes Mauricio, Pérez-Plasencia Carlos, and Madrid-Marina Vicente

 

12. Silencing of E6/E7 Expression in Cervical Cancer Stem-Like Cells

            Wenyi Gu, Nigel McMillan, and Chengzhong Yu

 

13. Two-Step Procedure for Evaluating Experimentally Induced DNA Damage: Texas Red and Comet Assays

            Gina J. Ferris, Lauren B. Shunkwiler, and Charles A. Kunos

 

14. Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe

            Colleen Rivard and Martina Bazzaro

 

Part V: Approaches to Detecting, Typing, and Staging of CxCA

 

15. Immunocytochemical Analysis of the Cervical Pap Smear

            Terry K. Morgan and Michelle Berlin

 

16. Diagnosis of HPV-Negative, Gastric-Type Adenocarcinoma of the Endocervix

            Edyta C. Pirog

 

17. Targeting of the HPV-16 E7 Protein by RNA Aptamers

            Julia Dolores Toscano-Garibay, María Luisa Benítez-Hess, and Luis Marat Alvarez-Salas

 

18. The Use of MYBL2 as a Novel Candidate Biomarker of Cervical Cancer

            Cara M. Martin, Katharine Astbury, Louise Kehoe, Jacqueline Barry O’Crowley, Sharon O’Toole, and John J. O’Leary

 

19. Fixation Methods for the Preservation of Morphology, RNAs, and Proteins in Paraffin-Embedded Human Cervical Cancer Cell Xenografts in Mice

            Yoko Matsuda and Toshiyuki Ishiwata

 

Part VI: Genomic Analyses of CxCA

 

20. Assessment of the HPV DNA Methylation Status in Cervical Lesions

            Mina Kalantari and Hans-Ulrich Bernard

 

21. MeDIP-on-Chip for Methylation Profiling

            Yaw-Wen Hsu, Rui-Lan Huang, and Hung-Cheng Lai

 

22. Use of DBD-FISH for the Study of Cervical Cancer Progression

            Eva I. Cortés-Gutiérrez, Jose L. Fernández, Martha I. Dávila-Rodríguez, Carmen López-Fernández, and Jaime Gosálvez

 

Part VII: Cellular Assays and Cell Culture Models of CxCA

 

23. A Quantitative and High-Throughput Assay of Human Papillomavirus DNA Replication

            David Gagnon, Amélie Fradet-Turcotte, and Jacques Archambault

 

24. Native Human Papillomavirus Production, Quantification, and Infectivity Analysis

            Jennifer Biryukov, Linda Cruz, Eric J. Ryndock, and Craig Meyers

 

25. Functional Analysis of HPV-Like Particle-Activated Langerhans Cells In Vitro

            Lisa Yan, Andrew W. Woodham, Diane M. Da Silva, and W. Martin Kast

 

26. Assessment of the Radiation Sensitivity of Cervical Cancer Cell Lines

            Mayil Vahanan Bose and Thangarajan Rajkumar

 

Part VIII: Animal Models of CxCA and Experimental Therapeutic Strategies

 

27. Mouse Model of Cervicovaginal Papillomavirus Infection

            Nicolas Çuburu, Rebecca J. Cerio, Cynthia D. Thompson, and Patricia M. Day

 

28. Establishment of Orthotopic Primary Cervix Cancer Xenografts

            Naz Chaudary, Karolina Jaluba, Melania Pintilie, and Richard P. Hill

 

29. Generation of K14-E7/∆N87ßcat Double Transgenic Mice as a Model of Cervical Cancer

            Gülay Bülüt and Aykut Üren