Cellular and Subcellular Nanotechnology: Methods and Protocols

ISBN-13: 9781627033350 / Angielski / Twarda / 2013 / 370 str.

Volkmar Weissig; Tamer Elbayoumi; Mark Olsen

Opis

In Cellular and Subcellular Nanotechnology: Methods and Protocols expert researchers in the field detail the most recent advances which have been made in utilizing the enormous potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and Practical, Cellular and Subcellular Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of applying nanotechnology to all areas of biomedical sciences.

Spis treści

1. Nanoparticle-GFP “Chemical Nose” Sensor for Cancer Cells Identification

Daniel F. Moyano and Vincent M. Rotello

2. A Method to Map Spatiotemporal pH Changes in a Multicellular Living Organism using a DNA Nanosensor

Sunaina Surana and Yamuna Krishnan

3. A Simple Method to Visualize and Assess the Integrity of Lysosomal Membrane in Mammalian Cells using a Fluorescent Dye

Syed K. Sohaebuddin and Liping Tang

4. Gold Nanoparticle as a Marker for Precise Localization of Nano-objects within Intracellular Sub-Domains

Valeriy Lukyanenko and Vadim Salnikov

5. Immunoisolation of Nanoparticles Containing Endocytic Vesicles for Drug Quantitation

Ari Nowacek, Irena Kadiu, JoEllyn McMillan and Howard E. Gendelman

6. Methods for Isolation and Identification of Nanoparticle-Containing Subcellular Compartments

Ari Nowacek, Irena Kadiu, JoEllyn McMillan and Howard E. Gendelman

7. Permeabilization of Cell Membrane for Delivery of Nano-objects to Cellular Sub-domains

Valeriy Lukyanenko

8. A Method to Encapsulate Molecular Cargo within DNA Icosahedra

Dhiraj Bhatia, Saikat Chakraborty, Shabana Mehtab and Yamuna Krishnan

9. Delivery of Plasmid DNA to Mammalian Cells using Polymer-Gold Nanorod Assemblies

James Ramos, Huang-Chiao Huang, and Kaushal Rege

10. Lipophilic Formulated Gold Porphyrin Nanoparticles for Chemotherapy

Puiyan Lee and Kenneth KY Wong

11. Mitochondria-specific Nano-Emulsified Therapy for Myocardial Protection against Doxorubicin-induced Cardiotoxicity

A.Faulk, V. Weissig and Tamer Elbayoumi

12. Formation of Pit-Spanning Phospholipid Bilayers on Nanostructured Silicon Dioxide Surfaces for Studying Biological Membrane Events

Indriati Pfeiffer and Michael Zäch

13. Characterization of Nanoparticle – Lipid Membrane Interactions using QCM-D

Rickard Frost and Sofia Svedhem

14. Single-Cell Nanosurgery

Maxwell B. Zeigler and Daniel T. Chiu

15. Single Quantum Dot Imaging in Living Cells

Jerry C. Chang and Sandra J. Rosenthal

16. Fabrication of Fluorescent Silica Nanoparticles with Aggregation-Induced Emission Luminogens for Cell Imaging

Sijie Chen, Jacky W. Y. Lam, and Ben Zhong Tang

17. Monitoring the Degradation of Reduction Sensitive Gene Carriers with Fluorescence Spectroscopy and Flow Cytometry

Constantin Hozsa, Miriam Breunig, and Achim Göpferich

18. Quantification of Intracellular Mitochondrial Displacements in Response to Nanomechanical Forces

Yaron R. Silberberg and Andrew E. Pelling

19. Imaging Select Mammalian Organelles Using Fluorescent Microscopy: Application to Drug Delivery

Paul D. R. Dyer, Arun K. Kotha, Marie W. Pettit, Simon C. W. Richardson

20. Real-Time Particle Tracking for Studying Intracellular Trafficking of Pharmaceutical Nanocarriers

Feiran Huang, Erin Watson, Christopher Dempsey, and Junghae Suh

21. Interactions of Nanoparticles with Proteins -Determination of Equilibrium Constants

Lennart Treuel, Marcelina Malissek

22. Tracing the Endocytic Pathways and Trafficking Kinetics of Cell Signaling Receptors using Single QD Nanoparticles

 Katye M. Fichter and Tania Q. Vu

 23. Cellular Internalization of Quantum DotsYue-Wern Huang, Han-Jung Lee, Betty Revon Liu, Huey-Jenn Chiang, Chi-Heng Wu

 

24. Electrochemical Scanning Tunneling Microscopy and Spectroscopy for Single Molecule Investigation

Andrea Alessandrini & Paolo Facci

25. Intracellular Delivery of Biologically Active Proteins with Peptide-based Carriers

S.L. Lo and S. Wang

26. Lipo-oligoarginine-based Intracellular Delivery

Jae Sam Lee and Ching-Hsuan Tung

27. Fluorescent Spherical Monodisperse Silica Core-shell Nanoparticles with a Protein-binding Biofunctional Shell

Achim Weber, Marion Herz and Günter E.M. Tovar

28. Direct Quantification of PTD Transduction Using Real-Time Monitoring

Mi-Sook Lee and Song Her

29. Genotoxic Assessment of Carbon Nanotubes

O. Nešković, G. Joksić, A. Valenta Šobot, J. Cvetićanin, Dj. Trpkov, A. Leskovac and S. Petrović

30. Separation Science: Principles and Applications for the Analysis of Bionanoparticles by Asymmetrical Flow Field-Flow Fractionation (AF4)

Alexandre Moquin, Françoise M. Winnik, and Dusica Maysinger

31. Polymersomes-mediated Delivery of Fluorescent Probes for Targeted and Long Term Imaging in Live Cell Microscopy                                                                                                                                                                    

Irene Canton and Giuseppe Battaglia

32. Protocol for the Preparation of Stimuli-responsive Gold Nanoparticles Capped with Elastin-based Pentapeptides

Blurb

In Cellular and Subcellular Nanotechnology: Methods and Protocols expert researchers in the field detail the most recent advances which have been made in utilizing the enormous potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and Practical, Cellular and Subcellular Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of applying nanotechnology to all areas of biomedical sciences.