1. Assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans Aggeliki Sotiriou, Christina Ploumi, Nikolaos Charmpilas and Nektarios Tavernarakis 2. Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino Suzita Mohd Noor, Chee Ern David Wong, Pooi-Fong Wong and Anwar Norazit 3. Methods for detection of mitochondrial reactive oxygen species in senescent cells Fenniche Salma, Oubaddou Yassire, Bakri Youssef, Dupuy Corinne and Ameziane El Hassani Rabii 4. Assessment of cell cycle progression and mitotic slippage by videomicroscopy Luca Mattiello, Sara Soliman Abdel Rehim, Gwenola Manic and Ilio Vitale 5. The original colorimetric method to detect cellular senescence Manjari Dimri and Goberdhan P. Dimri 6. Assessing microbiota composition in the context of aging Diana Campos-Iglesias, José M.P. Freije and Carlos López-Otín 7. Assessing chronological aging in Saccharomyces cerevisiae Adina Schulze, Andreas Zimmermann, Katharina Kainz, Nadine B. Egger, Maria A. Bauer, Frank Madeo and Didac Carmona-Gutierrez 8. Image processing and supervised machine learning for retinal microglia characterization in senescence Soyoung Choi, Daniel Hill, Jonathan Young and Maria Francesca Cordeiro 9. Measuring telomerase activity using TRAP assays Gabriele Saretzki 10. High throughput assessment of cellular senescence Giulia Cerrato, Allan Sauvat, Felix Peyre, Oliver Kepp and Guido Kroemer 11. Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events V. Paget, O. Guipaud, A. François and F. Milliat 12. Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP) Nicolas Malaquin and Francis Rodier 13. Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors Vanessa Klapp, Norma Bloy, Carlos Jiménez-Cortegana, Aitziber Buqué and Giulia Petroni 14. Mechanistic exploration of autophagy and aging by RNA interference Hui Pan, Oliver Kepp and Guido Kroemer