Cellular Senescence: Methods and Protocols

ISBN-13: 9781493989300 / Angielski / Twarda / 2018 / 253 str.

DeMaria, Marco

Opis

This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Spis treści

1. Detecting Cellular Senescence in Reprogramming

Coralie Cazin_, Mathieu von Joest, and Han Li

 

2. DNA Damage In situ Ligation followed by Proximity Ligation Assay (DI-PLA)

Alessandro Galbiati and Fabrizio d’Adda di Fagagna

 

3. Reactive Oxygen Species Detection in Senescent Cells

Stella Victorelli and João F. Passos

 

4. Cellular Identification and Quantification of Senescence-associated β-Galactosidase Activity in vivo

Bennett G. Childs, Tyler J. Bussian, and Darren J. Baker

  5. Relative Human Telomere Length Quantification by Real-time PCR Vasilishina A., Kropotov A., Spivak I., and Bernadotte A   6. Assessing Functional Roles of the Senescence-associated Secretory Phenotype (SASP)

Nicolas Malaquin, Véronique Tu, and Francis Rodier

 

7. Measuring the Inflammasome in Oncogene-induced Senescence

Irene Fernández-Duran, Núria Tarrats, Priya Hari, Juan Carlos Acosta

 

8. Alarmin Detection in Senescent Cells

Dong Eun Kim and Albert R. Davalos

 

9. IMR90 ER:RAS: A Cell Model of Oncogene-induced Senescence

Andrew J Innes and Jesús Gil

 

10. Genotoxic Stress-induced Senescence

Dorothy N.Y. Fan and Clemens A. Schmitt

 

11. A Multi-parametric Assay to Evaluate Senescent Cells

Hilah Gal, Ziv Porat, and Valery Krizhanovsky

 

12. A Novel Quantitative Method for the Detection of Lipofuscin, the Main Byproduct of Cellular Senescence, in Fluids

Sophia V Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, Iordanis Mourouzis, Sophia Havaki, Aikaterini Athanasiou, Panagiotis V.S. Vasileiou, Aggelos Margetis, Athanassios Kotsinas, Nikolaos G. Kastrinakis, Petros Sfikakis, Paul Townsend, Emmanuel Mikros, Constantinos Pantos, and Vassilis G. Gorgoulis

 

13. Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry

Christopher D. Wiley, Sonnet Davis, and Arvind Ramanathan

 

14. Quantification of Autophagy during Senescence

Joon Tae Park, Young-Sam Lee, and Sang Chul Park

15. Quantification of Aneuploidy in Mammalian Systems

Hilda van den Bos, Bjorn Bakker, Aaron Taudt, Victor Guryev, Maria Colomé-Tatché, Peter M. Lansdorp, Floris Foijer, and Diana C.J. Spierings

 

16. Identification of Genomic Alterations Through Multi-Level DNA Structural Analysis            

Ryan K. Shultzaberger and John Dresios

 

17. Mouse Models of Accelerated Cellular Senescence

Matthew J. Yousefzadeh, Kendra Melos, Luise Angelini, Christin E. Burd, Paul D. Robbins, and Laura J. Niedernhofer

 

18. Methods to Quantify the NF-kB Pathway during Senescence

Lei Zhang, Jing Zhao, Aditi Gurkar, Laura J. Niedernhofer, and Paul D. Robbins