Cellular Programming and Reprogramming: Methods and Protocols

ISBN-13: 9781607616900 / Angielski / Twarda / 2010 / 334 str.

Sheng Ding

Opis

Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body's own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome. In Cellular Programming and Reprogramming: Methods and Protocols, expert researchers cover the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes to highlight tips on troubleshooting and avoiding known pitfalls. Essential and cutting-edge, Cellular Programming and Reprogramming: Methods and Protocols promises to aid scientists attempting to advance stem cell biology in order to better treat devastating human diseases, including cardiovascular disease, neurodegenerative disease, musculoskeletal disease, diabetes, and cancer.

Spis treści

1. Human Embryonic Stem Cell Derivation, Maintenance, and Differentiation to Trophoblast Ge Lin, Kristen Martins-Taylor, and Ren-He Xu 2. Isolation and Maintenance of Mouse Epiblast Stem Cells Josh G. Chenoweth and Paul J. Tesar 3. Functional Assays for Hematopoietic Stem Cell Self-Renewal John M. Perry and Linheng Li 4. Isolation Procedure and Characterization of Multipotent Adult Progenitor Cells (MAPC) from Rat Bone Marrow Kartik Subramanian, Martine Geraerts, Karen A. Pauwelyn, Yonsil Park, D. Jason Owens, Manja Muijtjens, Fernando Ulloa-Montoya, Yeuhua Jiang, Catherine M. Verfaillie, and Wei-Shou Hu 5. Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In vitro Yan Shi 6. Mesoderm Cell Development from ES Cells Takumi Era 7. Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells Shi-Jiang Lu, Qiang Feng, Jennifer S. Park, and Robert Lanza 8. Directed Differentiation of Neural and Subtype Specific Neuronal Cells from hESCs Bao-Yang Hu and Su-Chun Zhang 9. Directing Human Embryonic Stem Cells to a Retinal Fate Deepak Lamba, Julianne Gust, and Thomas A. Reh 10. Bovine Somatic Cell Nuclear Transfer Pablo J. Ross and Jose B. Cibelli 11. Cell Fusion Induced Reprogramming Jeong Tae Do and Hans R. Schöler 12. An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells Prashant Mali, Zhaohui Ye, Bin-Kuan Chou, Jonathan Yen, and Linzhao Cheng 13. Using Small Molecules to Improve Generation of Induced Pluripotent Stem Cells from Somatic Cells Caroline Desponts and Sheng Ding 14. Reprogramming of Committed Lymphoid Cells by Enforced Transcription Factor Expression Huafeng Xie, Catherine V. Laiosa, andThomas Graf 15. Reprogramming of B Cells César Cobaleda 16. Adult Cell Fate Reprogramming: Converting Liver to Pancreas Irit Meivar-Levy and Sarah Ferber 17. In vitro Reprogramming of Pancreatic Cells to Hepatocytes Daniel Eberhard, Kathy O’Neill, Zoë D. Burke, and David Tosh 18. Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches Wenlin Li and Sheng Ding 19. Small Molecule Screen in Zebrafish and HSC Expansion Eirini Trompouki and Leonard I. Zon 20. Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation Kathleen M. Munson and Jing-Ruey J. Yeh

Blurb

Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body’s own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome. In Cellular Programming and Reprogramming: Methods and Protocols, expert researchers cover the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes to highlight tips on troubleshooting and avoiding known pitfalls.

Essential and cutting-edge, Cellular Programming and Reprogramming: Methods and Protocols promises to aid scientists attempting to advance stem cell biology in order to better treat devastating human diseases, including cardiovascular disease, neurodegenerative disease, musculoskeletal disease, diabetes, and cancer.