Cellular Heterogeneity: Methods and Protocols

ISBN-13: 9781493976799 / Angielski / Twarda / 2018 / 363 str.

Natasha S. Barteneva; Ivan A. Vorobjev

Spis treści

Part I: General

1. Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level

            Natasha S. Barteneva and Ivan A. Vorobjev

2. Integrating Analysis of Cellular Heterogeneity in High Content Dose-Response Studies

            Albert Gough, Tong Ying Shun, D. Lansing Taylor, and Mark Schurdak

3. Image-Based Tracking of Heterogeneous Single Cell Phenotypes

            Katherin Patsch, Shannon M. Mumenthaler, and Daniel Ruderman

Part II: Cytometry in Cellular Heterogeneity Research

4. High-Dimensional Single Cell Analysis with Mass Cytometry

            Tess Melinda Brodie and Vinko Tosevski

5. Spectral and Imaging Flow Cytometry in Phytoplankton Research

            Veronika Dashkova, Jeff Clapper, Ivan A. Vorobjev, and Natasha S. Barteneva

6. X-Ray Fluorescence Detected Flow Cytometry

            Andrew M. Crawford and James E. Penner-Hahn

7. Multiparametric Analysis of Myeloid Populations by Flow Cytometry

            Sara A. Mathie, Alastair L. Corbin, Hayley L. Eames, and Irina A. Udalova

8. Quantitation of IRF3 Nuclear Translocation in Heterogeneous Cellular Populations from Cervical Tissue Using Imaging Flow Cytometry

            Radiana T. Trifonova and Natasha S. Barteneva

9. Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry

            Ekaterina Kopeikina, Marina Dukhinova, and Eugene D. Ponomarev

10. Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System during Neuroinflammation and Neurodegeneration

            Marina Dukhinova, Ekaterina Kopeikina, and Eugene D. Ponomarev

Part III: Imaging and Spectroscopy Methods in Heterogeneity Studies

11. Analysis of Microtubule Dynamics Heterogeneity in Cell Culture

            Anara Serikbaeva, Anna Tvorogova, Sholpan Kauanova, and Ivan A. Vorobjev

12. Heterogeneity of Focal Adhesions and Focal Contacts in Motile Fibroblasts

            Aleena Gladkikh, Anastasia Kovaleva, Anna Tvorogova, and Ivan A. Vorobjev

13. Laser Tweezers Raman Micro-Spectroscopy of Single Cells and Biological Particles

            Maria Navas-Moreno and James W. Chan

Part VI: Metabolic and Molecular Biological Methods in Cell Heterogeneity Tracking

14. Quantification of the Metabolic Heterogeneity in Mycobacterial Cells Through the Measurement of the NADH/NAD⁺ Ratio Using a Genetically-Encoded Sensor

            Shabir Ahmad Bhat, Iram Khan Iqbal, and Ashwani Kumar

15. Characterizing Cell Heterogeneity Using PCR Fingerprinting of Surface Multigene Families in Protozoan Parasites

            Víctor Seco-Hidalgo, Antonio Osuna, and Luis Miguel de Pablos

16. Assessing Carbon Source-Dependent Phenotypic Variability in Pseudomonas putida

            Pablo I. Nikel and Víctor de Lorenzo

Part V: Chromatin and Cell Cycle Heterogeneity

17. The Retinal Pigment Epithelial Cell Line (ARPE-19) Displays Mosaic Structural Chromosomal Aberrations

            Elizaveta Fasler-Kan, Nijas Aliu, Kerstin Wunderlich, Sylvia Ketterer, Sabrina Ruggiero, Steffen Berger, and Peter Meyer

18. FACS Isolation of Viable Cells in Different Cell Cycle Stages from Asynchronous Culture for RNA-Sequencing

            Daria M. Potashnikova, Sergey A. Golyshev, Alexey A. Penin, Maria D. Logacheva, Anna V. Klepikova, Anastasia A. Zharikova, Alexey A. Mironov, Eugene V. Sheval, and Ivan A. Vorobjev

19. Measuring Nanoscale Chromatin Heterogeneity with Partial Wave Spectroscopic Microscopy

            Scott Gladstein, Andrew Stawarz, Luay M. Almassalha, Lusik Cherkezyan, John E. Chandler, Xiang Zhou, Hariharan Subramanian, and Vadim Backman

Blurb

This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 

Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.