Cell Viability Assays: Methods and Protocols

ISBN-13: 9781493969593 / Angielski / Twarda / 2017 / 299 str.

Daniel Gilbert; Oliver Friedrich

Opis

This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell Viability Assays: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Spis treści

1. Basic Colorimetric Proliferation Assays: MTT, WST and Resazurin

Konstantin Präbst, Hannes Engelhardt, Stefan Ringgeler, and Holger Hübner

 

2. Assaying Cellular Viability using the Neutral Red Uptake Assay

Gamze Ates, Tamara Vanhaecke, Vera Rogiers, Robim M. Rodrigues

 

3. Assessment of Cell Viability with Single, Dual, and Multi-Staining Methods using Image Cytometry

Leo Li-Ying Chan, Kelsey J. McCulley, and Sarah L. Kessel

 

4. High-throughput Spheroid Screens using Volume, Resazurin Reduction, and Acid Phosphatase Activity

Delyan P. Ivanov, Anna M. Grabowska, and Martin C. Garnett

 

5. A Protocol for in vitro High-throughput Chemical Susceptibility Screening in Differentiating NT2 Stem Cells

Ann-Katrin Menzner and Daniel F. Gilbert

 

6. Ferroptosis and Cell Death Analysis by Flow Cytometry

Daishi Chen, Ilker Y. Eyupoglu, and Nicolai Savaskan

 

7. Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness

Natalia Smolina, Joseph Bruton, Anna Kostareva, and Thomas Sejersen

 

8. An ATP-based Luciferase Viability Assay for Animal African Trypanosomes using a 96-well Plate

Keisuke Suganuma, Nthatisi Innocentia Molefe, and Noboru Inoue

 

9. SYBR^â Green I Based Fluorescence Assay to Assess Cell Viability of Malaria Parasites for Routine Use in Compound Screening

Maria Leidenberger, Cornelia Voigtländer, Nina Simon, and Barbara Kappes

 

10. Screening Applications to Test Cellular Fitness in Transwell^® Models after Nanoparticle Treatment

Bastian Christ, Christina Fey, Alevtina Cubukova, Heike Walles, Sofia Dembski, and Marco Metzger

 

11. Assays for Analyzing the Role of Transport Proteins in the Uptake and the Vectorial Transport of Substances Affecting Cell Viability

Emir Taghikhani, Martin F. Fromm, and Jörg König

 

12. Metabolite Profiling Of Mammalian Cell Culture Processes to Evaluate Cellular Viability

Isobelle M. Evie, Alan J. Dickson, and Mark Elvin

 

13. Assaying Spontaneous Network Activity and Cellular Viability using Multi-well Microelectrode Arrays

Jasmine P Brown, Brittany S Lynch, Itaevia M Curry-Chisolm, Timothy J Shafer,  and Jenna D Strickland

 

14. Quantitative Ratiometric Ca²⁺ Imaging to Assess Cell Viability

Oliver Friedrich and Stewart I. Head

 

15. Functional Viability: Measurement of Synaptic Vesicle Pool Sizes

Jana K. Wrosch and Teja W. Groemer

 

16. Phenotyping Cellular Viability by Functional Analysis of Ion Channels: GlyR-targeted Screening in NT2-N Cells

Katharina Kuenzel, Sepideh Abolpour Mofrad, and Daniel F. Gilbert

 

17. Systematic Cell-Based Phenotyping of Missense Alleles

Aenne S. Thormählen and Heiko Runz

 

18. Second Harmonic Generation Microscopy of Muscle Cell Morphology and Dynamics

Andreas Buttgereit and Friedrich-Alexander

 

19. Assessment of Population and ECM Production Using Multiphoton Microscopy as an Indicator of Cell Viability

M. Vielreicher and O. Friedrich

 

20. Average Rheological Quantities of Cells in Monolayers

Haider Dakhil  and Andreas Wierschem

 

21. Measurement of Cellular Behavior by Electrochemical Impedance Sensing

Simin Öz, Achim Breiling, and Christian Maercker

 

22. Nano-QSAR Model for Predicting Cell Viability of Human Embryonic Kidney Cells

Serena Manganelli and  Emilio Benfenati

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