Cell Viability Assays: Methods and Protocols

ISBN-13: 9781071630518 / Angielski / Twarda / 2023 / 618 str.

Oliver Friedrich; Daniel F. Gilbert

Opis

This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environments.

Spis treści

Part I: Single Cell Assays in 2D Environments

 

1. Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness

            Natalia Smolina, Aleksandr Khudiakov, and Anna Kostareva

 

2. The MTT Assay: A Method for Error Minimization and Interpretation in Measuring Cytotoxicity and Estimating Cell Viability

            Mahshid Ghasemi, Sisi Liang, Quang Minh Luu, and Ivan Kempson

 

3. Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions

            Daniel F. Gilbert, Oliver Friedrich, and Joachim Wiest

 

4. Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viability

            Jana Katharina Dahlmanns and Marc Dahlmanns

 

5. Assaying Mitochondrial Function by Multi-Parametric Flow Cytometry

            Hannah C. Alberico, Jonathan L. Tilly, and Dori C. Woods

 

6. High-Efficiency Single Cell Electrical Impedance Spectroscopy

            Yongxiang Feng, Liang Huang, Peng Zhao, Fei Liang, and Wenhui Wang

 

7. Cell Viability Multiplexing: Quantification of Cellular Viability by Barcode Flow Cytometry and Computational Analysis

            Valentina Giudice, Victoria Fonseca, Carmine Selleri, and Massimo Gadina

 

8. Average Rheological Quantities of Cells in Monolayers

            Santanu Kumar Basu, Haider Dakhil, and Andreas Wierschem

 

9. Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arrays

            Seline S. Choo, Jackson Y. Keever, Jasmine Brown, Jenna D. Strickland, and Timothy J. Shafer

 

10. Quantitative Live-Cell Ca²⁺ Imaging During Isotropic Cell Stretch

            Ulrike Schöler, Anna-Lena Merten, Sebastian Schürmann, and Oliver Friedrich

 

11. Assessment of Cell Viability in Electrically Excitable Muscle Cells through Intact Twitch Stimulation

            Julian Bauer, Stewart I. Head, and Oliver Friedrich

 

12. Evaluating Cellular Viability by iTRAQ Proteomic Profiling

            Anne Poljak, Mark Raftery, and Patsie Polly

 

13. Quantifying pH in Malaria Using pHluorin and Flow Cytometry

            Jeffrey Agyapong and Petra Rohrbach

 

14. Cell Membrane State, Permeability, and Elasticity Assessment for Single Cells and Cell Ensembles

            Nicolas Färber, Simon V. Neidinger, and Christoph Westerhausen

 

15. Neutral Red Uptake Assay to Assess Cytotoxicity In Vitro

            Robim M. Rodrigues, Marth Stinckens, Gamze Ates, and Tamara Vanhaecke

 

16. Digital Holographic Microscopy to Assess Cell Behavior

            Brad Bazow, Van K. Lam, Thuc Phan, Byung Min Chung, George Nehmetallah, and Christopher B. Raub

 

17. Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells

            Dominik Schneidereit, Stefanie Nübler, and Oliver Friedrich

 

18. Optimization of Cell Viability Assays for Drug Sensitivity Screens

            Peter Larsson and Toshima Z. Parris

 

19. Cellasys #8: A Microphysiometric Assay for Real-Time Cell Analysis within 24 Hours

            Sebastian Eggert, Svitlana Stetsenko, and Joachim Wiest

 

20. Live Cell Adhesion, Migration, and Invasion Assays

            Jordi Pijuan, Anna Macià, and Anaïs Panosa

 

21. Cell Viability and Immunogenic Function of T Cells Loaded with Nanoparticles for Spatial Guidance in Magnetic Fields

            Felix Pfister, Christoph Alexiou, and Christina Janko

 

Part II: Single Cell Assays in 3D Environments

 

22. Transwell In Vitro Cell Migration and Invasion Assays

            Calvin R. Justus, Mona A. Marie, Edward J. Sanderlin, and Li V. Yang

 

23. Live Cell Calcium Imaging of Non-Adherent Cells

            Lydia J. Bye, Rocio K. Finol-Urdaneta, and David J. Adams

 

24. FUCCI Reporter Gene-Based Cell Cycle Analysis

            Lena Fischer and Ingo Thievessen

 

25. Cell Viability Assays for 3D Cellular Constructs

            Zachary Congress, Matthew Brovold, and Shay Soker

 

26. Toxicological Analysis by Assessment of Vascularization and Cell Viability Using the Chicken's Chorioallantoic Membrane (CAM Assay)

            Nadine Wiesmann, Jürgen Brieger, and Jonas Eckrich

 

27. Assessment of Tissue Viability by Functional Imaging of Membrane Potential

            Callum M. Zgierski-Johnston and Peter Kohl

 

28. Multiplexed Viability Assays for High-Throughput Screening of Spheroids of Multiple Sizes

            Mohana Marimuthu and Thomas Gervais

 

29. Tracking Gut Motility in Organ and Cultures

            Peng Du, Vikram Joshi, and Arthur Beyder

 

30. Optimized Method of 3D Scaffold Seeding, Cell Cultivation, and Monitoring Cell Status for Bone Tissue Engineering

            Adrian Krolinski, Kai Sommer, Johanna Wiesner, Oliver Friedrich, and Martin Vielreicher