Cell Separation: Fundamentals, Analytical and Preparative Methods

ISBN-13: 9783642094545 / Angielski / Miękka / 2010 / 203 str.

Ashok Kumar; Igor Yu Galaev; Bo Mattiasson

Opis

Cell separation, which was once limited to merely being a basic technique for fractionating different cell populations, has come a long way in the last two decades. New, advanced and more speci?c and selective techniques have emerged as the demand for isolating a speci?c cell type for various biological applications has increased. Ef?cient and cost-effective techniques for fr- tionation and isolation of target cell types are necessary to provide pure cell populations for diagnostics, biotechnological and biomedical applications. One can see a considerable need, both in biomedical research and in di- nostic medicine, for the speci?c separation of a discrete population of cells from a mixture. For example, in the ?eld of tissue engineering, isolation of stemcellsfromtissuesororgansisofparticularlygreatimportance.Moreover, understanding cell developmental pathways becomes increasingly signi?cant as diagnosis and treatment of diseases turns more to the molecular level. The diagnosis of cell-related diseases requires methods of detection, isolation and theanalysisofindividualcells, regardlessoftheirrelativecontentinthetissue. Since cell-based therapies now turn towards more realistic medical options, developing an effective separation system for large-scale cell separation has becomechallengingresearchgoalforcellbiologistsandbiotechnologists.The ideal technique should provide in a short time a good yield of cells with high puritywhile maintaining cellfunction.Despite the growingneed formethods to separate cells into cell subpopulations, the existing cell-separation te- niques stillhave somelimitations when the desired degree ofperformance on apreparativescaleisrequired.Wewillseemoreresearchfocusinthisdirection in the future. The traditional techniques of micro?ltration, ultra?ltration and ultrac- trifugation, which exploit differences in cell size, shape and density, have remainedtheworkhorsesdespitelowspeci?cityandproblemswithscalingup

Spis treści

M. B. Dainiak, A. Kumar, I.Y. Galaev, B. Mattiasson: Methods in Cell Separations.- S.F. Ibrahim, G. van den Engh: Flow Cytometry and Cell Sorting.- A.A. Neurauter, M. Bonyhadi, E. Lien, L. Nøkleby, E. Ruud, S. Camacho, T. Aarvak: Cell Isolation and Expansion Using Dynabeads®.- J. Hubble: Affinity Adsorption of Cells to Surfaces and Strategies for Cell Detachment.- M.B. Dainiak, I.Y. Galaev, A. Kumar, F.M. Plieva, B. Mattiasson: Chromatography of Living Cells Using Supermacroporous Hydrogels, Cryogels.- R.E. Nordon, S. Craig: Hollow-Fibre Affinity Cell Separation.- J.M.S. Cabral: Cell Partitioning in Aqueous Two-Phase Polymer Systems.- M. Kamihira, A. Kumar: Development of Separation Technique for Stem Cells.-