Cell Senescence: Methods and Protocols

ISBN-13: 9781627032384 / Angielski / Twarda / 2013 / 538 str.

Lorenzo Galluzzi; Ilio Vitale; Oliver Kepp

Opis

Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In Cell Senescence: Methods and Protocols, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model organisms encompassing bacteria, fungi, worms, flies, zebrafish, and mammalian cells. These techniques cover the study of all the morphological, biochemical and functional manifestations of senescence at the cellular level and include protocols for population analyses and high-throughput approaches in suitable model organisms. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Spis treści

1. Cell Senescence as Both a Dynamic and a Static Phenotype

Andrew RJ Young, Masako Narita, and Masahi Narita

2. Senescence Regulation by mTOR

Vjekoslav Dulic

3. Senescence Regulation by the p53 Protein Family

Yingjuan Qian and Xinbin Chen

4. Markers of Cellular Senescence

Amancio Carnero

5. Biomarkers of Cell Senescence Assessed by Imaging Cytometry

Hong Zhao and Zbigniew Darzynkiewicz

6. Cytofluorometic Assessment of Cell Cycle Progression

Ilio Vitale, Mohamed Jemaa, Lorenzo Galluzzi, Didier Metivier, Maria Castedo and Guido Kroemer

7. Quantification of Cell Cycle-arresting Proteins

Oliver Kepp, Isabelle Martins, Laurie Menger, Mickael Michaud, Sandy Adjemian, Abdul Qader Sukkurwala, Lorenzo Galluzzi, and Guido Kroemer

8. Colorimetric Detection of Senescence-associated Beta Galactosidase (SA-Betagal)

9. Chemiluminescent Detection of Senescence-Associated Beta Galactosidase (SA-Betagal)

10. Detection of the Senescence-associated Secretory Phenotype(SASP)

Francis Rodier

11. Unbiased Characterization of the Senescence-associated Secretome using SILAC-Based Quantitative Proteomics

Juan Carlos Acosta, Ambrosius P. Snijders, and Jesus Gil

12. Detection of Senescence-associated Heterochromatin Foci (SAHF)

Katherine M. Aird and Rugang Zhang

13. Monitoring DNA Damage During Cell Senescence

Glyn Nelson and Thomas von Zglinicki

14. Assessment and Quantification of Telomerase Enzyme Activity

Michelle F. Maritz, Laura A. Richards, Karen L. MacKenzie

15. Methods for the Assessment of Telomere Status

Asako J. Nakamura

16. Detection of Nuclear Envelope Alterations in Senescence

Clea Barcena, Fernando G. Osorio and Jose M.P. Freije

17. Measuring Reactive Oxygen Species in Senescent Cels

Joao F. Passos, Satomi Miwa, and Thomas von Zglinicki

18. Quantification of Protein Carbonylation

Nancy D. Wehr and Rodney L. Levine

19. Assays for the Measurement of Lipid Peroxidation

Ana Cipak Gasparovic, Morana Jaganjac, Branka Mihaljevic, Suzana Borovic Sunjic, and Neven Zarkovic

20. Raman Spectroscopy for the Detection of AGEs/ALEs

J. Renwich Beattie, John J. McGarvey, Alan W. Stitt

21. Monitoring Oncogenic B-RAF-induced Senscence in Melanocytes

Sieu L. Tran and Helen Rizos

22. Methods to Investigate the Role of SIRT1 in Endothelial Senescence

Bo Bai and Yu Wang

23. Monitoring Nutrient Signaling Through the Longevity Protein p66^SHC1

Sofia Chiatamone Ranieri and Giovambattista Pani

24. Profiling the Metabolic Signature of Senescence

Florian M. Geier, Silke Fuchs, Gabriel Valbuena, Armand M. Leroi, and Jacob G. Bundy

25. Genome-wide RNAi Screening to Identify Regulators of Oncogene-induced Cellular Senescence

Narendra Wajapeyee, Sara Deibler, and Michael R. Gree

26. An Integrated Approach for Monitoring Cell Senescence

Tatiana V. Pospelova, Zhanna V. Chitikova, Valery A. Pospelov

27. Robust Multiparametric Assessment of Cellular Senescence

Clara Correia-Melo, Diana Jurk and Joao F. Passos

28. Assessing Chronological Aging in Bacteria

Stavros Gonidakis and Valter D. Longo

29. Assessing Organismal Aging in the Filamentous Fungas Podospora Anserina

Heinz D. Osiewacz, Andrea Hamann, Sandra Zintel

30. Assessing Chronological Aging in Saccharomyces cerevisiae

Jia Hu, Min Wei, Mario G. Mirisola, and Valter D. Longo

31. Assessing Aging and Senescent Decline in Caenorhabditis elegans: Cohort Survival Analysis

Eirini Lionaki and Nektarios Tavernarakis

32. High Throughput and Longitudinal Analysis of Aging and Senescent Decline in Caenorhabditis elegans

Eirini Lionaki and Nektarios Tavernarakis

33. Assessing Senescence in Drosophila using Video Tracking

Reza Ardekani, Simon Tavare and John Tower

34. Assessing Vascular Senescence in Zebrafish

Sandra Donnini, Antonio Giachetti, and Marian Ziche

Blurb

Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In Cell Senescence: Methods and Protocols, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model organisms encompassing bacteria, fungi, worms, flies, zebrafish, and mammalian cells. These techniques cover the study of all the morphological, biochemical and functional manifestations of senescence at the cellular level and include protocols for population analyses and high-throughput approaches in suitable model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.