Cell-Secreted Vesicles: Methods and Protocols

ISBN-13: 9781071632024 / Angielski

Seppo Vainio

Opis

This detailed volume presents hands-on technological protocols used to target an array of cell-secreted extracellular vesicles (EVs) in a variety of biological systems. Beginning with methods for EV purification and analysis, the book continues with sections on the study of EV functions as well as specific systems and models allowing for the study of EVs of different origin. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cell-Secreted Vesicles: Methods and Protocols serves as an ideal guide to conducting systematic assays in an effort to further our understanding of the mode of assembly, secretion, and targeting of EVs which will serve eventually as new therapeutic openings.

Spis treści

Part I: Purification and Analysis of Single or Multiple EVs

 

1. Plasmon-Enhanced Characterization of Single Extracellular Vesicles

            Mi Ho Jeong, Taehwang Son, and Hyungsoon Im

 

2. Detection of Cell-Derived Exosomes via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles

            Yang Li, Yunpeng Wang, Jinwei Tian, and Jian-An Huang

 

3. In Vivo Analysis of Heterogeneous Extracellular Vesicles Using a Red-Shifted Bioluminescence Resonance Energy Transfer Reporter Protein

            Gloria I. Perez, Michael H. Bachmann, and Masamitsu Kanada

 

4. Characterization of Extracellular Vesicles by Transmission Electron Microscopy and Immunolabelling Electron Microscopy

            Maribel Lara Corona, Ilse Hurbain, Graça Raposo, and Guillaume Van Niel

 

5. Extracellular Vesicles Isolation by a Tangential-Flow-Filtration-Based Large-Scale Purification Method

            Runjie Yuan, Yijun Zhou, Gabriel F. Arias, and Dirk P. Dittmer

 

6. Metabolomics Analysis of Urinary Extracellular Vesicles by Nuclear Magnetic Resonance and Liquid Chromatography-Mass Spectrometry

            Marta Martin-Lorenzo, Dolores Molero, Gloria Alvarez-Llamas

 

7. Methodologies for Scalable Production of High Quality Purified Small Extracellular Vesicles from Conditioned Medium

            Gabriella Andriolo, Elena Provasi, Andrea Brambilla, Stefano Panella, Sabrina Soncin, Viviana Lo Cicero, Marina Radrizzani, Lucia Turchetto, and Lucio Barile

 

8. Automated Online Isolation and Fractionation Method for Subpopulations of Extracellular Vesicles

            Susanne K. Wiedmer, Evgen Multia, Thanaporn Liangsupree, and Marja-Liisa Riekkola

 

Part II: Targeting Cell Behavior Functions of EVs

 

9. A Novel Assay for Investigating the Role of Exosomes in Tumor Cell-Endothelial Cell Crosstalk

            Yan Qiu, Wenli Jiang, and Ye Zeng

 

10. Nanoparticle (NP) Loading by Direct Incubation with Extracellular Vesicles-Secretor Cells: NP Encapsulation and Exosome Characterization

            Ana Martín-Pardillos and Pilar Martín-Duque

 

11. Two Complementary Strategies to Quantitate Extracellular Vesicles Uptake Using Bioluminescence and Non-Lipidic Dyes

            Víctor Toribio and María Yáñez-Mó

 

12. Integrin-Mediated Exosomal Homing to Organs

            Eun Jeong Park and Motomu Shimaoka

 

13. Methods and Protocols for Using Extracellular Vesicles as Delivery Vehicles in Neuronal Research

            Manjusha Vaidya, Nasif Sayeed, Caroline Hobson, Sandeep Sreerama, Jonhoi Smith, Riya Shah, and Kiminobu Sugaya

 

14. Quantitative Analysis of Extracellular Vesicle Release Using Artificial MicroRNAs

            Albert Lu

 

Part III: Systems and Models to Study EVs

 

15. Purification of Bacterial-Enriched Extracellular Vesicle Samples from Feces by Density Gradient Ultracentrifugation

            Nadiya Byts, Olha Makieieva, Artem Zhyvolozhnyi, Genevieve Bart, Johanna Korvala, Jenni Hekkala, Sonja Salmi, Anatoliy Samoylenko, and Justus Reunanen

 

16. Isolation and Characterization of Extracellular Vesicles from Lymphocytes

            Lujain Al-Ghadir and Zhi Chen

 

17. Biogenesis of Mesoporous Silica Nanoparticles Enclosed in Extracellular Vesicles by Mouse Renal Adenocarcinoma Cells

            Feby Wijaya Pratiwi, Keerthanaa Balasubramanian Shanthi, Olha Makieieva, Zih An Chen, Artem Zhyvolozhnyi, Ilkka Miinalainen, Genevieve Bart, Anatoliy Samoylenko, and Si-Han Wu

 

18. Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications

            Mireia Bernuz, Arnau Pallarès-Rusiñol, Rosanna Rossi, Carolina Fernández-Senac, Mercè Martí, and María Isabel Pividori

 

19. Cilia-Derived Extracellular Vesicles in Caenorhabditis elegans: In Vivo Imaging and Quantification of Extracellular Vesicles Release and Capture

            Adrià Razzauti, Teresa Lobo, and Patrick Laurent

 

20. Exosome-Based COVID-19 Vaccine

            Jaeyoung Kim and Nikita Thapa