Cell-Penetrating Peptides: Methods and Protocols

ISBN-13: 9781493949625 / Angielski / Miękka / 2016 / 472 str.

Ulo Langel

Opis

Divided into three parts this volume summarizes the most important areas of Cell-Penetrating Peptides (CPP) research . Part one briefly presents the historical background of CPP studies and the classifications of the available CPPs, and then summarizes the approaches for prediction of novel CPPs. Part two mainly describes the methods for studies of "naked" CPPs, that is, CPPs without conjugated cargos. Last but not least part three presents a representative and brief summary of functionality issues of CPPs, both in vitro and in vivo. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Concise and easy-to-use, Cell-Penetrating Peptides: Methods and Protocols, Second Edition hopes to raise relevant questions for further development.

Spis treści

Part I: Introduction

1. Classes of Cell-Penetrating Peptides

Margus Pooga and Ülo Langel

 

2. Penetratin Story: An Overview

Edmond Dupont, Alain Prochiantz, and Alain Joliot

 

3. Prediction of Cell-Penetrating Peptides

Mattias Hällbrink and Mati Karelson

 

4. Computer-Aided Virtual Screening and Designing Of Cell-Penetrating Peptides

Ankur Gautam, Kumardeep Chaudhary, Rahul Kumar, and Gajendra Pal Singh Raghava

 

Part II: Methods To Test Mechanisms Of Cell-Penetrating Peptides

5. Investigating Membrane Interactions and Structures Of Cpps

Fatemeh Madani and Astrid Gräslund

 

6. Determining the Effects Of Membrane-Interacting Peptides on Membrane Integrity

William C. Wimley

 

7. Study of CPP Mechanisms By Mass-Spectrometry

Sandrine Sagan, Chérine Bechara, and Fabienne Burlina

 

8. Methods to Study the Role of the Glycocalyx in the Uptake of Cell Penetrating Peptides

Samuel Schmidt, Rike Wallbrecher, Toin H. Van Kuppevelt,  and Roland Brock

9. Toxicity, Immunogenicity, Uptake and Kinetics Methods for Cpps

Julia Uusna, Kent Langel, and Ülo Langel

 

10. Unravelling the Mechanisms Of Peptide-Mediated Delivery of Nucleic Acids Using Electron Microscopy

Helerin Margus, Carmen Juks, and Margus Pooga

 

11. SCARA Involvement in the Uptake of Nanoparticles Formed by Cell Penetrating Peptides

 Henrik Helmfors, Staffan Lindberg, and Ülo Langel 

 

 

Part III: Applications Of Cpps In Vitro And In Vivo

12. Protein Mimicry and the Design of Bioactive Cell Penetrating Peptides

John Howl and Sarah Jones

 

13. Pepducins and Other Lipidated Peptides as Mechanistic Probes and Therapeutics

Ping Zhang, Lidija Covic, and Athan Kuliopulos

 

14. Identification and Characterization of Homing Peptides Using In Vivo Peptide Phage Display

Maija Hyvönen and Pirjo Laakkonen

 

15. Antiviral and Antimicrobial Applications Of Cell Penetrating Peptides
Kalle Pärn, Elo Eriste, and Ülo Langel

16. Visualising Actin Architectures in Cells Incubated with Cell Penetrating Peptides

Lin He, Peter D. Watson, and Arwyn T. Jones

 

17. Cell-Penetrating Peptides as Carriers For Transepithelial Drug Delivery In Vitro

Stine Rønholt, Mie Kristensen, and Hanne Mørck Nielsen

        

18. A Pathway Toward Tumor Cell Selective

Cpps?

Isabel D. Alves, Manon Carré. and Solange Lavielle

 

19. Characterization of Cellular Internalization Pathways for CPP-Mediated Oligonucleotide Delivery Pepfects and Nickfects

Piret Arukuusk, Ly Pärnaste, Mattias Hällbrink, and Ülo Langel

 

20. In Vitro Assays to Assess Exon Skipping in Duchenne Muscular Dystrophy

Prisca Boisguerin, Liz O'Donovan, Michael J. Gait, and Bernard Lebleu

 

21. Applications of Apob LDLR Binding Domain Approach for the Development of CNS Penetrating Peptides for Alzheimer’s Disease

Eliezer Masliah and Brian Spencer

 

22. Utilization of Cpps for In Vivo Delivery of Bioactive Cargo - The Effect Of Cargo Type, CPP-Cargo Association Method and Formulation

Kaido Kurrikoff, Kadi-Liis Veiman, and Ülo Langel

 

23. Application of Cpps for Brain Delivery

Artita Srimanee , Jakob Regberg, and Ülo Langel

 

24. Intracellular Delivery of Nanoparticles with Cell Penetrating Peptides

Giuseppina Salzano and Vladimir  P. Torchilin

25. Multifunctional Oligoaminoamides for The Receptor-Specific Delivery of Therapeutic RNA

Judith Weber, Ulrich Lächelt, and Ernst Wagner

 

26. Cell Penetrating Peptides for Chemical Biological Studies

Ikuhuko Nakase, Toshihide Takeuch, and Shiroh Futaki

27. Experiences with CPP-Based Self Assembling Peptide Systems for Topical Delivery of Botulinum Toxin

Jane Lee, Phil Kennedy and Jacob M. Waugh

 

28. Applications of Cpps in Gene Modulation of Plants

Alicja Ziemienowicz, Jordan Pepper, and François Eudes

 

29. DNA Transfer Into Animal Cells Using Stearyled CPP Based Transfection Reagent

Kristiina Karro, Tiiu Männik, Andres Männik, and Mart Ustav

 

30. Live Cell Genomics: Cell-Specific Transcriptome Capture in Live Tissues and Cells

Thomas Bell and James Eberwine

 

31. Live Cell Genomics:  RNA Exon-Specific RNA Binding Protein Isolation

Thomas Bell and James Eberwine