Cell Migration: Developmental Methods and Protocols

ISBN-13: 9781617792069 / Angielski / Twarda / 2011 / 476 str.

Claire M. Wells; Maddy Parsons

Opis

Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study. The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances. Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D. It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for any researcher who wishes to explore this significant area of cell biology.

Spis treści

1. Cell Migration: An Overview

            Miguel Vicente-Manzanares and Alan Rick Horwitz

 

2. Scratch-Wound Assay

            Giles Cory

 

3. HGF-Induced DU145 Cell Scatter Assay

            Sally T. Fram, Claire M. Wells, and Gareth E. Jones

 

4. Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time

            Sanjay Chaubey, Anne J. Ridley, and Claire M. Wells

 

5. Imaging Cells Within 3D Cell Derived Matrix

            Samantha J. King and Maddy Parsons

 

6. Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro

            Daniel M. Suter

 

7. Boyden Chamber

            Marco Falasca, Claudio Raimondi, and Tania Maffucci

 

8. Transwell® Invasion Assays

            John Marshall

 

9. Imaging Podosome Dynamics and Matrix Degradation

            Taylor W. Starnes, Christa L. Cortesio, and Anna Huttenlocher

 

10. Endothelial Cell Migration Under Flow

            Beata Wojciak-Stothard

 

11. In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments

            Michael Sixt

 

12. Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy

            Robert J. Cain, Bárbara Borda d’Água, and Anne J. Ridley

 

13. Chemotaxis of Slow Migrating Mammalian Cells Analyzed by Video Microscopy

            Roman Zantl and Elias Horn

 

14. Live Cell Fluorescence Microscopy Techniques

            Shawn A. Galdeen and Alison J. North

 

15. Measuring Invasion in an Organotypic Model

            Veronika Jenei, Maria L. Nystrom, and Gareth J. Thomas

 

16. Analysis of Cell Migration Using Caenorhabditis elegans as a Model System

            Ming-Ching Wong, Maria Martynovsky, and Jean E. Schwarzbauer

 

17. Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration

            Carolina G.A. Moreira, Jennifer C. Regan, Anna Zaidman-Rémy, Antonio Jacinto,  and Soren Prag

 

18. Measuring Inflammatory Cell Migration in the Zebrafish

            Philip M. Elks, Catherine A. Loynes, and Stephen A. Renshaw

 

19. Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement

            Mohit Prasad, Xiaobo Wang, Li He, and Denise J. Montell

 

20. Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants

            Yulia Artemenko, Kristen F. Swaney, and Peter N. Devreotes

 

21. Simple Experimental and Spontaneous Metastasis Assays in Mice

            Gary M. Box and Suzanne A. Eccles

 

22. Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour

of Cancer Cells In Vivo

            Sylvia E. Le Dévédec, Wies van Roosmalen, Chantal Pont, Reshma Lalai, Hans de Bont, and Bob van de Water

 

23. Measuring Angiogenesis in Mice

            Bernardo Tavora and Kairbaan Hodivala-Dilke

 

24. Time-Lapse Imaging of Chick Cardiac Precursor Cells

            Junfang Song, Qiaoyun Yue, and Andrea Münsterberg

 

25. ­Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy

            Juliane P. Schwarz, Ireen König, and Kurt I. Anderson

 

26. Fluorescence Recovery After Photobleaching

            Alex Carisey, Matthew Stroud, Ricky Tsang, and Christoph Ballestrem

 

27. Measuring FRET Using Time-Resolved FLIM

            Penny E. Morton and Maddy Parsons

 

28. Cell Migration in Confinement: A Micro-Channel Based Assay

            Mélina L. Heuzé, Olivier Collin, Emmanuel Terriac, Ana-Maria Lennon-Duménil, and Matthieu Piel

 

29. Functional Screening with a Live Cell Imaging Based Random Cell Migration Assay

            Wies van Roosmalen, Sylvia E. Le Dévédec, Sandra Zovko, Hans de Bont, and Bob van de Water

 

30. Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays

            Eric Theveneau and Roberto Mayor