Cell Imaging Techniques: Methods and Protocols

ISBN-13: 9781493962464 / Angielski / Miękka / 2016 / 550 str.

Douglas J. Taatjes; Jurgen Roth

Opis

This contribution to the Methods in Molecular Biology collection has been updated to include new chapters covering colocalization and laser microdissection, among other emerging techniques. It features the series trademark of clear and reproducible protocols.

Recenzje

From the book reviews:

"The different techniques are described in deep details, the chapters having an abstract, introduction, materials, methods, and, last but not least, notes; each method is described step by step with very, very useful links to the notes. ... should be read not only by very specialized scientists, but also by those who usually work with classic microscopic methods, to open their minds and increase their appetite for more precise and complex measurements done on different level of complex biological structures." (Ioan I. Ardelean, Bulletin of Micro and Nanoelectrotechnologies, Vol. 5 (3-4), December, 2014)

"The goal is to provide seasoned microscopists with a list of protocols to add sophistication to their existing microscopy regimen. The intended audience is experienced researchers who are familiar with imaging in a core microscopy facility. ... this book is more useful to microscopists who routinely use microscopic techniques in their research and are knowledgeable about the recent advances in technology. ... an ideal reference for a core microscope facility. Each chapter is written independently by a different group of researchers and can stand alone." (Latha Malaiyandi, Doody's Book Reviews, December, 2013)

Spis treści

1.  Digital Images are Data – and Should be Treated as Such

Douglas W. Cromey

 2. Epi-fluorescence Microscopy

Donna J. Webb and Claire M. Brown

3. Live-Cell Migration and Adhesion Turnover Assays

Lacoste, J. Young, K. and Brown, C. M.

4. Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of HemostaticProteins in Human Venous Valves

Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill

5. Colocalization Analysis in Fluorescence Microscopy

Jeremy Adler

6. A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown

Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman

7. Light Sheet Microscopy in Cell Biology

Raju Tomer, Khaled Khairy and Philipp J. Keller

 8. Image-based High-throughput Screening for Inhibitors of Angiogenesis

Lasse Evensen, Wolfgang Link, and James B. Lorens

9. Intravital Microscopy to Image Membrane Trafficking in Live Rats

Andrius Masedunskas, Monika Sramkova, Laura Parente and Roberto Weigert

10. Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential Interference Contrast Microscopy and Planar Illumination Microscopy

Wei Sun, Lehui Xiao, and Ning Fang

 11. Laser Scanning Cytometry. Principles and Applications: An Update

Piotr Pozarowski , Elena Holden, and Zbigniew Darzynkiewicz

12. Laser Capture Microdissection for Protein and NanoString RNA Analysis

Yelena Golubeva, Rosalba Salcedo, Claudius Mueller, Lance A. Liotta, and Virginia Espina

 ^{13. Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy}

^{Anthony S. Quinn,  Jacob H. Rand,  Xiao-Xuan Wu,  and Douglas J. Taatjes}

 ^{14. Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy}

^{Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko}

 ^{15. Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation}

^{Joanna L. MacKay and Sanjay Kumar}

16. Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells

Lilia A. Chtcheglova and Peter Hinterdorfer

 17. Porosome: The Secretory NanoMachine in Cells

Bhanu P. Jen

 18. Stereology and Morphometry of Lung Tissue

Christian Mühlfeld, Lars Knudsen, Matthias Ochs

 19. A Novel Combined Imaging/Stereological Method for the Analysis of Human Sural Nerve Biopsies for Clinical Diagnosis

Michele A. VonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes

 20. Correlative Light–Electron Microscopy as a Tool to Study In vivo Dynamics and uUtrastructure of Intracellular Structures

Elena V. Polishchuk, Roman S. Polishchuk and Alberto Luini

21. Photooxidation Technology for Correlative Light and Electron Microscopy

Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, Margit Pavelka, Adolf Ellinger

 22. Electron Microscopy of Endocytic Pathways

Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, Margit Pavelka

 23. Morphological Analysis of Autophagy

Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori

 24. Cytochemical Detection of Peroxisomes and Mitochondria

Nina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader

 25. Histochemical Detection of Lipid Droplets in Cultured Cells

Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto

 26. Environmental Scanning Electron Microscopy in Cell Biology

J. E. McGregor, L. T. L. Staniewicz,  S. E. Guthrie (neé Kirk) and A. M. Donald

 27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell Biology

F. Rosso, F. Papale and A. Barbarisi

 28. High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular Organelles

Paul Walther, Eberhard Schmid, Katharina Höhn

 29. MALDI Imaging Mass Spectrometry for Direct Tissue Analysis

Stephan Meding and Axel Walch

 27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell Biology

F. Rosso, F. Papale and A. Barbarisi

 28. High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular Organelles

Paul Walther, Eberhard Schmid, Katharina Höhn

 29. MALDI Imaging Mass Spectrometry for Direct Tissue Analysis

Stephan Meding and Axel Walch

 27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell Biology

F. Rosso, F. Papale and A. Barbarisi

 28. High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular Organelles

Paul Walther, Eberhard Schmid, Katharina Höhn

 29. MALDI Imaging Mass Spectrometry for Direct Tissue Analysis

Stephan Meding and Axel Walch

 27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell Biology

F. Rosso, F. Papale and A. Barbarisi

 28. High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular Organelles

Paul Walther, Eberhard Schmid, Katharina Höhn

 29. MALDI Imaging Mass Spectrometry for Direct Tissue Analysis

Stephan Meding and Axel Walch

 27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell Biology

F. Rosso, F. Papale and A. Barbarisi

 28. High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular Organelles

Paul Walther, Eberhard Schmid, Katharina Höhn

 29. MALDI Imaging Mass Spectrometry for Direct Tissue Analysis

Stephan Meding and Axel Walch

Blurb

Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters  covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

 Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.