Cell Electrophoresis

ISBN-13: 9780849389184 / Angielski / Twarda / 1994 / 336 str.

Johann Bauer; Bauer Bauer

Opis

This book presents a summary of the application and instrumentation of cell electrophoresis. The method of making cell purification and characterization possible according to the cellular negative surface charge density is discussed, and ideas for future developments are explained. The negative electrostatic forces at cell surfaces provide information about cell-cell interaction, blood vessel sealing, cytokine actions, cell transformation, ion transport phenomena, and other biological phenomena. Recalculations of the physical principles of cell electrophoresis reveal possibilities for removing disruptive factors caused by electrical current, heat, and sedimentation. The introduction of computer technology, the performance of simultaneous two-parameter measurements, and the application of cell-friendly but current-inert buffer systems render the method more reliable and efficient.

Spis treści

Theoretical Considerations:Contributions to a Mathematical Theory of Free Flow Electrophoresis, F.G. BoeseNumerical Description of Zone Electrophoresis in the CFE Device, T.M. Grateful, A.M. Athalye, and E.N. LightfootImprovements of the Method:Theoretical and Experimental Studies on the Stabilization of Hydrodynamic Flow in Free-Fluid Electrophoresis, P.H. Rhodes and R.S. SnyderThe Loading and Unloading of Cells in Electrophoretic Separations, P. ToddElectrode Compartment Separating Membranes for Cell Electrophoresis, J. Heinrich and H. WagnerCell Electrophoresis Using Antibodies and Antigens as Ligands, H.H.P. Cohly and S.K. DasBuffers and Their Effects on Cells:New Buffer Systems for Cell Electrophoresis, K.A. Kinsley and L.S. RodkeyAlteration of Cellular Features after Exposure to Low Ionic Strength Medium, I. BernhardtElectrophoresis of Cells at Physiologic Ionic Strength, M.V. GolovanovThe Importance of Determination of the Negative Surface Charge Density of Cells:Simultaneous Two-Parameter Measurements of the Electrophoretic Features of Cell Populations and Their Sedimental Characteristics, G.G. SlivinskyCell Interactions and Surface Hydrophilicity: Influence of Lewis Acid-Base and Electrostatic Forces, C. J. van OssElectrical Surface Phenomena of Endothelial Cells, F.F. VargasApplication of Cell Electrophoresis for Clinical Diagnosis, W. Schütt, N. Hashimoto, and M. ShimizuThe Negative Surface Charge Density of Cells and Their Actual State of Differentiation or Activation, J. BauerCell Electrophoresis in Space:Cell Electrophoresis in Microgravity: Past and Future, D.R. MorrisonCell Electrophoresis in Microgravity, U. Friedrich, G. Ruyters, and J. Bauer

O autorze

Johann Bauer