Cell Cycle Synchronization: Methods and Protocols

ISBN-13: 9781493982486 / Angielski / Miękka / 2018 / 354 str.

Gaspar Banfalvi

Spis treści

Preface...
Table of Contents...
Contributors...

Part I Cell Cycle Synchronization

1. Overview of Cell Synchronization

Gaspar Banfalvi 

 

Part II Physical Fractionation

2. Synchronization of  Mammalian Cells and Nuclei by Centrifugal Elutriation
Gaspar Banfalvi

3. Image Cytofluorometry for the Quantification of Ploidy and Endoplasmic Reticulum Stress in Cancer Cells

Laura Senovilla, Yohann Demont, Juliette Humeau, Norma Bloy, and Guido Kroemer

4. Large Scale Mitotic Cell Synchronization

Kalyan Dulla and Anna Santamaria

Part III Chemical Blockade

5. Synchronization and Desynchronization of Cells by Interventions on the Spindle Assembly Checkpoint

Mohamed Jemaà, Gwenola Manic, and Ilio Vitale

 

6. Synchronization of Mammalian Cell Cultures by Serum Deprivation
Thomas J. Langan, Kyle R. Rodgers, and Richard C. Chou

7. DNA Damage Response Resulting from Replication Stress Induced by Synchronization of Cells by Inhibitors of DNA Replication: Analysis by  Flow Cytometry
H. Dorota Halicka, Hong Zhao , Jiangwei Li, Jorge Garcia, Monika Podhorecka, and Zbigniew Darzynkiewicz

8. Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias Latipes
Takashi Iwamatsu

9. Flow Cytometry Analysis of Cell Cycle and Specific Cell Synchronization with Butyrate
Cong-Jun Li

10. Chemically Induced Cell Cycle Arrest in Perfusion Cell Culture
Gabor Nagy, Bence Tanczos, Eszter Fidrus, Laszlo Talas and Gaspar Banfalvi

11. Analysis of Nuclear Uracil DNA – Glycosylase (nUDG) Turnover During the Cell Cycle
Jennifer A. Fischer and Salvatore Caradonna

12. Synchronization of  Hela Cells
Hoi Tang Ma and Randy Y.C. Poon


Part IV Synchronization of Unicellular Organisms

13. Synchronization of Bacillus Subtilis Cells by Spore Germination and Outgrowth
Gaspar Banfalvi

14. Synchronization of Yeast
Jessica Smith, Arkadi Manukyan, Hui Hua, Huzefa Dungrawala, and Brandt L. Schneider

15. Synchronization of  Pathogenic Protozoans
Staffan Swärd and Karin Troell


Part V Synchronizing Mammalian and Transfected Cells

16. Synchronization of In Vitro Maturation in Porcine Oocytes
Tamas Somfai and Yuhi Hirao


Part VI Synchronization of Plant Cells

17. Detection of Changes in the Medicago sativa Retinoblastoma-Related Protein (MsRBR1)
Phosphorylation during Cell Cycle Progression in Synchronized Cell Suspension Culture
Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, and Gábor V. Horváth


Part VII Synchronization of Embryonic Cells

18. Nuclear Treatment and Cell Cycle Synchronization for the Purpose of Mammalian and Primate Somatic Cell Nuclear Transfer (SCNT)
Yoel Sufaro and Benjamin E. Reubinoff


Part VIII Hematopoietic Stem Cells

19. Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation
Kap-Hyoun Ko, Robert Nordon, Tracey A. O’Brien, Geoff Symonds, and Alla Dolnikov


Part IX Clinical Study

20. Intracellular Flow Cytometry Improvements in Clinical Studies
Julie Demaret, Morgane Gossez, Fabienne Venet, and Guillaume Monneret


Part X Cell Cycle Control

21. Molecular Network Dynamics of Cell Cycle Control: Periodicity of Start and Finish
Alida Palmisano, Judit Zámborszky, Cihan Oguz, and Attila Csikász-Nagy

Blurb

This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in Cell Cycle Synchronization: Methods and Protocols, Second Edition cover subjects such as: physical fractionations including centrifugal elutriation of healthy and apoptotic cells, and nuclei of mammalian cells; large scale mitotic cell synchronization; chromosome formation during fertilization in eggs; synchronization of unicellular organisms; hematopoietic stem cells used to improve the engraftment in transplantation; and cell cycle control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Cell Cycle Synchronization: Methods and Protocols, Second Edition is a valuable resource for PhD students and postdoctoral fellows, and researchers interested in general science, pharmacy, medicine and public health, computer science, and life sciences. Specialists and professionals in cell biology, genetics, molecular biology, biochemistry, and pharmacology will also find this book useful.