Cell Cycle Synchronization: Methods and Protocols

ISBN-13: 9781493961948 / Angielski / Miękka / 2016 / 295 str.

Gaspar Banfalvi

Opis

This Methods in Molecular Biology(TM) volume offers detailed protocols, with theoretical background, and step-by-step instructions on implementing synchronization, as well as current techniques for the enhanced study of regulatory mechanisms in cell cycle events.

Spis treści

1. Overview of Cell Synchronization

            Gaspar Banfalvi

 

2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation

            Gaspar Banfalvi

 

3. Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells

            Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà, Santiago Rello-Varona, and Guido Kroemer

 

4. Large Scale Mitotic Cell Synchronization

            Kalyan Dulla and Anna Santamaria

 

5. Synchronization of Mammalian Cell Cultures by Serum Deprivation

            Thomas J. Langan and Richard C. Chou

 

6. Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry

            Zbigniew Darzynkiewicz, H. Dorota Halicka, Hong Zhao, and Monika Podhorecka

 

7. Chromosome Formation during Fertilization in Eggs of the Teleost, Oryzias latipes

            Takashi Iwamatsu

 

8. Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis

            Congjun Li

 

9. Analysis of Nuclear Uracil DNA–Glycosylase (nUDG) Turnover during the Cell Cycle

            Jennifer A. Fischer and Salvatore Caradonna

 

10. Synchronization of HeLa Cells

            Hoi Tang Ma and Randy Y.C. Poon

 

11. Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth

            Gaspar Banfalvi

 

12. Synchronization of Yeast

            Arkadi Manukyan, Lesley Abraham, Huzefa Dungrawala, and Brandt L. Schneider

 

13. Synchronization of Pathogenic Protozoans

            Staffan Svärd and Karin Troell

 

14. Synchronization of In Vitro Maturation in Porcine Oocytes

            Tamas Somfai and Yuji Hirao

 

15. Synchronization of Medicago sativa Cell Suspension Culture

            Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, and Gábor V. Horváth

 

16. Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)

            Yoel Shufaro  and Benjamin E. Reubinoff

 

17. Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation

            Kap-Hyoun Ko, Robert Nordon, Tracey A. O’Brien, Geoff Symonds, and Alla Dolnikov

 

18. Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients

            Fabienne Venet, Caroline Guignant, and Guillaume Monneret

 

19. Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish

            Attila Csikász-Nagy, Alida Palmisano, and Judit Zámborszky

Blurb

To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe.  In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events.  Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Detailed and easy to follow,  Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.